Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 9709 | 3' | -60.4 | NC_002577.1 | + | 136971 | 0.7 | 0.49457 |
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Target: 5'- -cCCUcGGACgUUCCGGAaGAGGCCGc -3' miRNA: 3'- uuGGA-CCUGgGAGGCCUgUUCCGGCc -5' |
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| 9709 | 3' | -60.4 | NC_002577.1 | + | 160366 | 0.7 | 0.49457 |
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Target: 5'- -cCCUcGGACgUUCCGGAaGAGGCCGc -3' miRNA: 3'- uuGGA-CCUGgGAGGCCUgUUCCGGCc -5' |
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| 9709 | 3' | -60.4 | NC_002577.1 | + | 160440 | 0.66 | 0.762174 |
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Target: 5'- cGCaaaGGGCCCgcaCGGGCucgggagaacuugcGGGGCCGGg -3' miRNA: 3'- uUGga-CCUGGGag-GCCUG--------------UUCCGGCC- -5' |
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| 9709 | 3' | -60.4 | NC_002577.1 | + | 161028 | 0.69 | 0.58063 |
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Target: 5'- cGCCUGGGCgggCCGGugGAGagcggcGCCGGa -3' miRNA: 3'- uUGGACCUGggaGGCCugUUC------CGGCC- -5' |
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| 9709 | 3' | -60.4 | NC_002577.1 | + | 161501 | 0.66 | 0.746424 |
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Target: 5'- cAGCCUccucGGCCCUuguggCCGGGCGccagAGGCCGc -3' miRNA: 3'- -UUGGAc---CUGGGA-----GGCCUGU----UCCGGCc -5' |
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| 9709 | 3' | -60.4 | NC_002577.1 | + | 162436 | 0.68 | 0.639769 |
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Target: 5'- gGACaaUGGAgCCUCCcacggcaaagucGGACGGGGCCGc -3' miRNA: 3'- -UUGg-ACCUgGGAGG------------CCUGUUCCGGCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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