Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9725 | 3' | -56.9 | NC_002577.1 | + | 159058 | 0.66 | 0.881431 |
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Target: 5'- cCGCCGGccGC-GGCCgcgccGUCCUCCg -3' miRNA: 3'- aGCGGCU--CGaCCGGaaca-UAGGAGGg -5' |
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| 9725 | 3' | -56.9 | NC_002577.1 | + | 138280 | 0.66 | 0.881431 |
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Target: 5'- cCGCCGGccGC-GGCCgcgccGUCCUCCg -3' miRNA: 3'- aGCGGCU--CGaCCGGaaca-UAGGAGGg -5' |
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| 9725 | 3' | -56.9 | NC_002577.1 | + | 38483 | 0.67 | 0.851951 |
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Target: 5'- gCGaCGGGUUGGUa--GUcgCCUCCCa -3' miRNA: 3'- aGCgGCUCGACCGgaaCAuaGGAGGG- -5' |
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| 9725 | 3' | -56.9 | NC_002577.1 | + | 128739 | 0.67 | 0.84961 |
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Target: 5'- gCGCCGAuGCUcGGCCg---GUucuucgcccagagcCCUCCCa -3' miRNA: 3'- aGCGGCU-CGA-CCGGaacaUA--------------GGAGGG- -5' |
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| 9725 | 3' | -56.9 | NC_002577.1 | + | 4875 | 0.67 | 0.84961 |
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Target: 5'- gCGCCGAuGCUcGGCCg---GUucuucgcccagagcCCUCCCa -3' miRNA: 3'- aGCGGCU-CGA-CCGGaacaUA--------------GGAGGG- -5' |
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| 9725 | 3' | -56.9 | NC_002577.1 | + | 122889 | 0.68 | 0.816811 |
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Target: 5'- aUGCCGAGCUcGGCCaguucgaugcccucgGUAcCCaUCCCc -3' miRNA: 3'- aGCGGCUCGA-CCGGaa-------------CAUaGG-AGGG- -5' |
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| 9725 | 3' | -56.9 | NC_002577.1 | + | 10725 | 0.68 | 0.816811 |
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Target: 5'- aUGCCGAGCUcGGCCaguucgaugcccucgGUAcCCaUCCCc -3' miRNA: 3'- aGCGGCUCGA-CCGGaa-------------CAUaGG-AGGG- -5' |
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| 9725 | 3' | -56.9 | NC_002577.1 | + | 99153 | 0.68 | 0.809053 |
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Target: 5'- aCGCUGGGCaacucGGCCaucgacccUCCUCCCa -3' miRNA: 3'- aGCGGCUCGa----CCGGaacau---AGGAGGG- -5' |
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| 9725 | 3' | -56.9 | NC_002577.1 | + | 59224 | 1.11 | 0.00187 |
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Target: 5'- uUCGCCGAGCUGGCCUUGUAUCCUCCCc -3' miRNA: 3'- -AGCGGCUCGACCGGAACAUAGGAGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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