Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9731 | 3' | -53.2 | NC_002577.1 | + | 135952 | 0.66 | 0.977709 |
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Target: 5'- --gAAAACCcCGCGGC-CGGCGaCAUc -3' miRNA: 3'- agaUUUUGGaGCGCUGcGCCGCaGUG- -5' |
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| 9731 | 3' | -53.2 | NC_002577.1 | + | 161386 | 0.66 | 0.977709 |
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Target: 5'- --gAAAACCcCGCGGC-CGGCGaCAUc -3' miRNA: 3'- agaUUUUGGaGCGCUGcGCCGCaGUG- -5' |
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| 9731 | 3' | -53.2 | NC_002577.1 | + | 95740 | 0.66 | 0.977709 |
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Target: 5'- uUCUGGggUCUCGaUGcCGUgaacucaagucGGCGUCACu -3' miRNA: 3'- -AGAUUuuGGAGC-GCuGCG-----------CCGCAGUG- -5' |
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| 9731 | 3' | -53.2 | NC_002577.1 | + | 119273 | 0.66 | 0.980001 |
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Target: 5'- cCUAAuguACCggCaCGugGUGuGCGUCACg -3' miRNA: 3'- aGAUUu--UGGa-GcGCugCGC-CGCAGUG- -5' |
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| 9731 | 3' | -53.2 | NC_002577.1 | + | 136274 | 0.66 | 0.980001 |
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Target: 5'- uUCggc-GCUgacCGCGGC-CGGCGUCAUg -3' miRNA: 3'- -AGauuuUGGa--GCGCUGcGCCGCAGUG- -5' |
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| 9731 | 3' | -53.2 | NC_002577.1 | + | 161064 | 0.66 | 0.980001 |
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Target: 5'- uUCggc-GCUgacCGCGGC-CGGCGUCAUg -3' miRNA: 3'- -AGauuuUGGa--GCGCUGcGCCGCAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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