Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9731 | 3' | -53.2 | NC_002577.1 | + | 122023 | 0.73 | 0.729169 |
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Target: 5'- --cGAAACCUaCGCGuucgcCGCGGCGUC-Cg -3' miRNA: 3'- agaUUUUGGA-GCGCu----GCGCCGCAGuG- -5' |
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| 9731 | 3' | -53.2 | NC_002577.1 | + | 75679 | 0.73 | 0.719177 |
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Target: 5'- gUCUcaag---CGCGAUGCGGCGUCGCc -3' miRNA: 3'- -AGAuuuuggaGCGCUGCGCCGCAGUG- -5' |
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| 9731 | 3' | -53.2 | NC_002577.1 | + | 11590 | 0.74 | 0.678544 |
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Target: 5'- --cGAAACCUaCGCGucgcCGCGGCGUC-Cg -3' miRNA: 3'- agaUUUUGGA-GCGCu---GCGCCGCAGuG- -5' |
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| 9731 | 3' | -53.2 | NC_002577.1 | + | 161643 | 0.84 | 0.220042 |
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Target: 5'- aUUGGGACCgUCGCGACGCGGUccaGUCGCg -3' miRNA: 3'- aGAUUUUGG-AGCGCUGCGCCG---CAGUG- -5' |
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| 9731 | 3' | -53.2 | NC_002577.1 | + | 135695 | 0.84 | 0.220042 |
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Target: 5'- aUUGGGACCgUCGCGACGCGGUccaGUCGCg -3' miRNA: 3'- aGAUUUUGG-AGCGCUGCGCCG---CAGUG- -5' |
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| 9731 | 3' | -53.2 | NC_002577.1 | + | 75125 | 1.11 | 0.004443 |
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Target: 5'- cUCUAAAACCUCGCGACGCGGCGUCACg -3' miRNA: 3'- -AGAUUUUGGAGCGCUGCGCCGCAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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