Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 9731 | 3' | -53.2 | NC_002577.1 | + | 16932 | 0.66 | 0.972554 |
|
Target: 5'- -gUGAGACgCUCGCuGCGCGGCa---- -3' miRNA: 3'- agAUUUUG-GAGCGcUGCGCCGcagug -5' |
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| 9731 | 3' | -53.2 | NC_002577.1 | + | 11590 | 0.74 | 0.678544 |
|
Target: 5'- --cGAAACCUaCGCGucgcCGCGGCGUC-Cg -3' miRNA: 3'- agaUUUUGGA-GCGCu---GCGCCGCAGuG- -5' |
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| 9731 | 3' | -53.2 | NC_002577.1 | + | 10585 | 0.68 | 0.947816 |
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Target: 5'- uUCggccGCgUCGcCGACGCGGgGUCGg -3' miRNA: 3'- -AGauuuUGgAGC-GCUGCGCCgCAGUg -5' |
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| 9731 | 3' | -53.2 | NC_002577.1 | + | 9894 | 0.66 | 0.970852 |
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Target: 5'- cUCUAuu-CCcCGCauuaccacauggacgGGcCGCGGCGUCGCg -3' miRNA: 3'- -AGAUuuuGGaGCG---------------CU-GCGCCGCAGUG- -5' |
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| 9731 | 3' | -53.2 | NC_002577.1 | + | 4802 | 0.67 | 0.951621 |
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Target: 5'- ---cAAACgUCGgacgggaCGGCGCGGCGUCGu -3' miRNA: 3'- agauUUUGgAGC-------GCUGCGCCGCAGUg -5' |
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| 9731 | 3' | -53.2 | NC_002577.1 | + | 546 | 0.71 | 0.822808 |
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Target: 5'- gUUAGGGCCUCuCGGgGuCGGCGUCAg -3' miRNA: 3'- aGAUUUUGGAGcGCUgC-GCCGCAGUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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