Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 9731 | 3' | -53.2 | NC_002577.1 | + | 546 | 0.71 | 0.822808 |
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Target: 5'- gUUAGGGCCUCuCGGgGuCGGCGUCAg -3' miRNA: 3'- aGAUUUUGGAGcGCUgC-GCCGCAGUg -5' |
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| 9731 | 3' | -53.2 | NC_002577.1 | + | 137763 | 0.7 | 0.878408 |
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Target: 5'- ----cGACCgcgUGCGcCGgGGCGUCGCg -3' miRNA: 3'- agauuUUGGa--GCGCuGCgCCGCAGUG- -5' |
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| 9731 | 3' | -53.2 | NC_002577.1 | + | 55039 | 0.69 | 0.898995 |
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Target: 5'- aUUUuGAGCCUCauCGGCuGCGGCGUCGg -3' miRNA: 3'- -AGAuUUUGGAGc-GCUG-CGCCGCAGUg -5' |
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| 9731 | 3' | -53.2 | NC_002577.1 | + | 138104 | 0.69 | 0.903496 |
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Target: 5'- aUCUAugucacgaaguuucGGAgCUCGCacaguucgccgGGCGCGGCcGUCACg -3' miRNA: 3'- -AGAU--------------UUUgGAGCG-----------CUGCGCCG-CAGUG- -5' |
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| 9731 | 3' | -53.2 | NC_002577.1 | + | 55104 | 0.68 | 0.943362 |
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Target: 5'- aCUAuuuGGACCUCGCGAcuCGCaGCGcUACc -3' miRNA: 3'- aGAU---UUUGGAGCGCU--GCGcCGCaGUG- -5' |
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| 9731 | 3' | -53.2 | NC_002577.1 | + | 136274 | 0.66 | 0.980001 |
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Target: 5'- uUCggc-GCUgacCGCGGC-CGGCGUCAUg -3' miRNA: 3'- -AGauuuUGGa--GCGCUGcGCCGCAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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