Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 9731 | 3' | -53.2 | NC_002577.1 | + | 17033 | 0.7 | 0.863554 |
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Target: 5'- gUCUaAAAGCgUgGCGGCGCGGC-UCAa -3' miRNA: 3'- -AGA-UUUUGgAgCGCUGCGCCGcAGUg -5' |
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| 9731 | 3' | -53.2 | NC_002577.1 | + | 159575 | 0.7 | 0.878408 |
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Target: 5'- ----cGACCgcgUGCGcCGgGGCGUCGCg -3' miRNA: 3'- agauuUUGGa--GCGCuGCgCCGCAGUG- -5' |
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| 9731 | 3' | -53.2 | NC_002577.1 | + | 62001 | 0.69 | 0.892365 |
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Target: 5'- aCUAAAAucguCCUCG-GGCGCGcCGUCGCc -3' miRNA: 3'- aGAUUUU----GGAGCgCUGCGCcGCAGUG- -5' |
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| 9731 | 3' | -53.2 | NC_002577.1 | + | 159234 | 0.69 | 0.903496 |
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Target: 5'- aUCUAugucacgaaguuucGGAgCUCGCacaguucgccgGGCGCGGCcGUCACg -3' miRNA: 3'- -AGAU--------------UUUgGAGCG-----------CUGCGCCG-CAGUG- -5' |
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| 9731 | 3' | -53.2 | NC_002577.1 | + | 68030 | 0.68 | 0.928019 |
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Target: 5'- aCUGAAugCggcaggCGCGugGCugugcaugucuacGGCGUCAa -3' miRNA: 3'- aGAUUUugGa-----GCGCugCG-------------CCGCAGUg -5' |
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| 9731 | 3' | -53.2 | NC_002577.1 | + | 75125 | 1.11 | 0.004443 |
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Target: 5'- cUCUAAAACCUCGCGACGCGGCGUCACg -3' miRNA: 3'- -AGAUUUUGGAGCGCUGCGCCGCAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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