Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9736 | 3' | -54.4 | NC_002577.1 | + | 98395 | 0.66 | 0.958261 |
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Target: 5'- gAGCCGcGUCUCcgagagCGUAuAUGGagUGGCCu- -3' miRNA: 3'- -UCGGC-CAGAGa-----GCAU-UACU--ACCGGcg -5' |
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| 9736 | 3' | -54.4 | NC_002577.1 | + | 90633 | 0.67 | 0.926639 |
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Target: 5'- uGCCaGUUUUUUGUucgGcUGGCCGCg -3' miRNA: 3'- uCGGcCAGAGAGCAuuaCuACCGGCG- -5' |
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| 9736 | 3' | -54.4 | NC_002577.1 | + | 41762 | 0.67 | 0.921167 |
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Target: 5'- gGGCCGGUCacgCUUc---UGuugGGCCGCg -3' miRNA: 3'- -UCGGCCAGa--GAGcauuACua-CCGGCG- -5' |
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| 9736 | 3' | -54.4 | NC_002577.1 | + | 105008 | 0.67 | 0.921167 |
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Target: 5'- uGGCCGGUCUucgauaggCUCG-AAUGGcucugaaGGUCGCc -3' miRNA: 3'- -UCGGCCAGA--------GAGCaUUACUa------CCGGCG- -5' |
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| 9736 | 3' | -54.4 | NC_002577.1 | + | 4643 | 0.68 | 0.89023 |
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Target: 5'- aGGCCGGgccg-CGcugcaggGAUGGCCGCg -3' miRNA: 3'- -UCGGCCagagaGCauua---CUACCGGCG- -5' |
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| 9736 | 3' | -54.4 | NC_002577.1 | + | 128970 | 0.68 | 0.89023 |
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Target: 5'- aGGCCGGgccg-CGcugcaggGAUGGCCGCg -3' miRNA: 3'- -UCGGCCagagaGCauua---CUACCGGCG- -5' |
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| 9736 | 3' | -54.4 | NC_002577.1 | + | 106434 | 0.69 | 0.868904 |
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Target: 5'- aAGCC-GUCcUUUGuUAAUGggGGCCGCg -3' miRNA: 3'- -UCGGcCAGaGAGC-AUUACuaCCGGCG- -5' |
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| 9736 | 3' | -54.4 | NC_002577.1 | + | 83210 | 0.72 | 0.734294 |
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Target: 5'- uGGCCGGUCUCgcaacgau-UGGCCGUg -3' miRNA: 3'- -UCGGCCAGAGagcauuacuACCGGCG- -5' |
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| 9736 | 3' | -54.4 | NC_002577.1 | + | 94018 | 1.11 | 0.003388 |
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Target: 5'- cAGCCGGUCUCUCGUAAUGAUGGCCGCc -3' miRNA: 3'- -UCGGCCAGAGAGCAUUACUACCGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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