miRNA: 3'- -UGCGGG-GUGaCGG-CGUCCu----GGCCaCC- -5'
miRNA: 3'- uGCGGGGUGa-----------CGGCGu-----CCUGGCCACC- -5'
miRNA: 3'- -UGCGGGGUGACGGCGUCCUGGCCACC- -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9737 | 5' | -62.9 | NC_002577.1 | + | 163259 | 0.71 | 0.360661 |
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Target: 5'- aGCGCCCgCGCgGCCgGCGGGcauguCCGGcGGg -3' miRNA: 3'- -UGCGGG-GUGaCGG-CGUCCu----GGCCaCC- -5' |
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| 9737 | 5' | -62.9 | NC_002577.1 | + | 136333 | 0.72 | 0.316112 |
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Target: 5'- cCGCCUCGCcuaccggagagcGCCGCcugggcGGGCCGGUGGa -3' miRNA: 3'- uGCGGGGUGa-----------CGGCGu-----CCUGGCCACC- -5' |
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| 9737 | 5' | -62.9 | NC_002577.1 | + | 100760 | 1.08 | 0.00097 |
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Target: 5'- uACGCCCCACUGCCGCAGGACCGGUGGc -3' miRNA: 3'- -UGCGGGGUGACGGCGUCCUGGCCACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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