Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9738 | 3' | -56.6 | NC_002577.1 | + | 42115 | 0.67 | 0.856775 |
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Target: 5'- cGCCcUGgUGGGuc-UCCaGACGGCCACg -3' miRNA: 3'- -UGGcAUgACCCucuAGG-CUGUCGGUG- -5' |
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| 9738 | 3' | -56.6 | NC_002577.1 | + | 42158 | 0.67 | 0.840992 |
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Target: 5'- uACCGacguugcuUACuuUGGGAGcGUCUGACGGUUACg -3' miRNA: 3'- -UGGC--------AUG--ACCCUC-UAGGCUGUCGGUG- -5' |
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| 9738 | 3' | -56.6 | NC_002577.1 | + | 5611 | 0.67 | 0.832816 |
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Target: 5'- gGCCGUGCcGGGAuAUCagaGGaAGCCGCu -3' miRNA: 3'- -UGGCAUGaCCCUcUAGg--CUgUCGGUG- -5' |
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| 9738 | 3' | -56.6 | NC_002577.1 | + | 105957 | 0.69 | 0.722971 |
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Target: 5'- gGCCGcGCUGGGaAGG-CCGGCccgucguguGGCCAUg -3' miRNA: 3'- -UGGCaUGACCC-UCUaGGCUG---------UCGGUG- -5' |
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| 9738 | 3' | -56.6 | NC_002577.1 | + | 86330 | 0.7 | 0.713102 |
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Target: 5'- uCCGUuuGCUa-GAGAUCUGACGGCCAa -3' miRNA: 3'- uGGCA--UGAccCUCUAGGCUGUCGGUg -5' |
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| 9738 | 3' | -56.6 | NC_002577.1 | + | 81176 | 0.7 | 0.703166 |
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Target: 5'- cCCGUAgaGGGAGuGUCUG-CAGUCGCc -3' miRNA: 3'- uGGCAUgaCCCUC-UAGGCuGUCGGUG- -5' |
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| 9738 | 3' | -56.6 | NC_002577.1 | + | 128002 | 0.7 | 0.69317 |
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Target: 5'- gGCCGUGCcGGGAuAUCagagGAgAGCCGCu -3' miRNA: 3'- -UGGCAUGaCCCUcUAGg---CUgUCGGUG- -5' |
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| 9738 | 3' | -56.6 | NC_002577.1 | + | 142204 | 0.71 | 0.622269 |
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Target: 5'- gUgGUGgUGGGAGAgaaauaCCGACGGCCGg -3' miRNA: 3'- uGgCAUgACCCUCUa-----GGCUGUCGGUg -5' |
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| 9738 | 3' | -56.6 | NC_002577.1 | + | 155135 | 0.71 | 0.622269 |
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Target: 5'- gUgGUGgUGGGAGAgaaauaCCGACGGCCGg -3' miRNA: 3'- uGgCAUgACCCUCUa-----GGCUGUCGGUg -5' |
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| 9738 | 3' | -56.6 | NC_002577.1 | + | 101198 | 1.1 | 0.002346 |
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Target: 5'- gACCGUACUGGGAGAUCCGACAGCCACg -3' miRNA: 3'- -UGGCAUGACCCUCUAGGCUGUCGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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