Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9741 | 3' | -53.1 | NC_002577.1 | + | 109392 | 0.67 | 0.971942 |
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Target: 5'- aGUGU--GCCGGC-UUCGGGucgagCGGg -3' miRNA: 3'- gUACGagUGGCCGuAAGCCCuua--GCC- -5' |
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| 9741 | 3' | -53.1 | NC_002577.1 | + | 134258 | 0.66 | 0.977165 |
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Target: 5'- aCAUGCcCGCCGGCcgcgCGGGcgcUCGc -3' miRNA: 3'- -GUACGaGUGGCCGuaa-GCCCuu-AGCc -5' |
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| 9741 | 3' | -53.1 | NC_002577.1 | + | 163070 | 0.66 | 0.977166 |
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Target: 5'- aCAUGCcCGCCGGCcgcgCGGGcgcUCGc -3' miRNA: 3'- -GUACGaGUGGCCGuaa-GCCCuu-AGCc -5' |
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| 9741 | 3' | -53.1 | NC_002577.1 | + | 147724 | 0.66 | 0.981636 |
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Target: 5'- gGUGgagCGUCGGCg--CGGGAAUCGGu -3' miRNA: 3'- gUACga-GUGGCCGuaaGCCCUUAGCC- -5' |
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| 9741 | 3' | -53.1 | NC_002577.1 | + | 49755 | 0.66 | 0.983608 |
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Target: 5'- uGUGCUCuACa-GCGUgCGGGAAgggCGGu -3' miRNA: 3'- gUACGAG-UGgcCGUAaGCCCUUa--GCC- -5' |
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| 9741 | 3' | -53.1 | NC_002577.1 | + | 66867 | 0.66 | 0.983608 |
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Target: 5'- -uUGCUCGCCGGUuguUUCGaacGAcgUGGc -3' miRNA: 3'- guACGAGUGGCCGu--AAGCc--CUuaGCC- -5' |
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| 9741 | 3' | -53.1 | NC_002577.1 | + | 157081 | 0.66 | 0.983608 |
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Target: 5'- --cGCUCGauuuaCGGCGggcUGGGAcUCGGu -3' miRNA: 3'- guaCGAGUg----GCCGUaa-GCCCUuAGCC- -5' |
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| 9741 | 3' | -53.1 | NC_002577.1 | + | 140257 | 0.66 | 0.983608 |
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Target: 5'- --cGCUCGauuuaCGGCGggcUGGGAcUCGGu -3' miRNA: 3'- guaCGAGUg----GCCGUaa-GCCCUuAGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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