Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9741 | 3' | -53.1 | NC_002577.1 | + | 43419 | 0.7 | 0.869995 |
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Target: 5'- uCAUGUacgaUCACCGGCAg--GGGGAUCc- -3' miRNA: 3'- -GUACG----AGUGGCCGUaagCCCUUAGcc -5' |
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| 9741 | 3' | -53.1 | NC_002577.1 | + | 4588 | 0.71 | 0.826862 |
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Target: 5'- gCGUGCUCuccuacgGCCGacucuugggagcguGCAUcguUCGGGggUCGGc -3' miRNA: 3'- -GUACGAG-------UGGC--------------CGUA---AGCCCuuAGCC- -5' |
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| 9741 | 3' | -53.1 | NC_002577.1 | + | 129026 | 0.71 | 0.826862 |
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Target: 5'- gCGUGCUCuccuacgGCCGacucuugggagcguGCAUcguUCGGGggUCGGc -3' miRNA: 3'- -GUACGAG-------UGGC--------------CGUA---AGCCCuuAGCC- -5' |
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| 9741 | 3' | -53.1 | NC_002577.1 | + | 125668 | 0.72 | 0.776637 |
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Target: 5'- --cGCguaagUACCGGCGUUCcGGGcGGUCGGc -3' miRNA: 3'- guaCGa----GUGGCCGUAAG-CCC-UUAGCC- -5' |
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| 9741 | 3' | -53.1 | NC_002577.1 | + | 7946 | 0.72 | 0.776637 |
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Target: 5'- --cGCguaagUACCGGCGUUCcGGGcGGUCGGc -3' miRNA: 3'- guaCGa----GUGGCCGUAAG-CCC-UUAGCC- -5' |
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| 9741 | 3' | -53.1 | NC_002577.1 | + | 124705 | 0.73 | 0.738155 |
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Target: 5'- --aGCUCACCGGCcccgCGGGAGaCGc -3' miRNA: 3'- guaCGAGUGGCCGuaa-GCCCUUaGCc -5' |
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| 9741 | 3' | -53.1 | NC_002577.1 | + | 8909 | 0.73 | 0.738154 |
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Target: 5'- --aGCUCACCGGCcccgCGGGAGaCGc -3' miRNA: 3'- guaCGAGUGGCCGuaa-GCCCUUaGCc -5' |
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| 9741 | 3' | -53.1 | NC_002577.1 | + | 111865 | 1.1 | 0.005349 |
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Target: 5'- aCAUGCUCACCGGCAUUCGGGAAUCGGc -3' miRNA: 3'- -GUACGAGUGGCCGUAAGCCCUUAGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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