Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 9741 | 3' | -53.1 | NC_002577.1 | + | 139551 | 0.67 | 0.968422 |
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Target: 5'- cCGUGCUUuCCGGCGUgcgcgccgccgcCGGGcg-CGGg -3' miRNA: 3'- -GUACGAGuGGCCGUAa-----------GCCCuuaGCC- -5' |
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| 9741 | 3' | -53.1 | NC_002577.1 | + | 140200 | 0.7 | 0.869995 |
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Target: 5'- --cGCUCGCCGGUGcccUUCcuacuGGGAUCGGc -3' miRNA: 3'- guaCGAGUGGCCGU---AAGc----CCUUAGCC- -5' |
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| 9741 | 3' | -53.1 | NC_002577.1 | + | 140257 | 0.66 | 0.983608 |
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Target: 5'- --cGCUCGauuuaCGGCGggcUGGGAcUCGGu -3' miRNA: 3'- guaCGAGUg----GCCGUaa-GCCCUuAGCC- -5' |
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| 9741 | 3' | -53.1 | NC_002577.1 | + | 147724 | 0.66 | 0.981636 |
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Target: 5'- gGUGgagCGUCGGCg--CGGGAAUCGGu -3' miRNA: 3'- gUACga-GUGGCCGuaaGCCCUUAGCC- -5' |
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| 9741 | 3' | -53.1 | NC_002577.1 | + | 157081 | 0.66 | 0.983608 |
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Target: 5'- --cGCUCGauuuaCGGCGggcUGGGAcUCGGu -3' miRNA: 3'- guaCGAGUg----GCCGUaa-GCCCUuAGCC- -5' |
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| 9741 | 3' | -53.1 | NC_002577.1 | + | 157138 | 0.7 | 0.869996 |
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Target: 5'- --cGCUCGCCGGUGcccUUCcuacuGGGAUCGGc -3' miRNA: 3'- guaCGAGUGGCCGU---AAGc----CCUUAGCC- -5' |
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| 9741 | 3' | -53.1 | NC_002577.1 | + | 157786 | 0.67 | 0.968422 |
|
Target: 5'- cCGUGCUUuCCGGCGUgcgcgccgccgcCGGGcg-CGGg -3' miRNA: 3'- -GUACGAGuGGCCGUAa-----------GCCCuuaGCC- -5' |
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| 9741 | 3' | -53.1 | NC_002577.1 | + | 163070 | 0.66 | 0.977166 |
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Target: 5'- aCAUGCcCGCCGGCcgcgCGGGcgcUCGc -3' miRNA: 3'- -GUACGaGUGGCCGuaa-GCCCuu-AGCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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