Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9742 | 3' | -48.4 | NC_002577.1 | + | 92259 | 0.66 | 0.999144 |
Target: 5'- ---uGGGCCGCAGUAGUUCCc----- -3' miRNA: 3'- auucCUUGGUGUCAUCAAGGaaucca -5' |
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9742 | 3' | -48.4 | NC_002577.1 | + | 55768 | 0.66 | 0.998439 |
Target: 5'- -uAGGAGCCuCAGUAGacacgCCggGGGa -3' miRNA: 3'- auUCCUUGGuGUCAUCaa---GGaaUCCa -5' |
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9742 | 3' | -48.4 | NC_002577.1 | + | 116864 | 0.68 | 0.993885 |
Target: 5'- -uGGGAA-CACcGUGGUUCCU-AGGUa -3' miRNA: 3'- auUCCUUgGUGuCAUCAAGGAaUCCA- -5' |
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9742 | 3' | -48.4 | NC_002577.1 | + | 116682 | 0.69 | 0.985961 |
Target: 5'- -uAGGAACCACGGU-GUUCCcacGGc -3' miRNA: 3'- auUCCUUGGUGUCAuCAAGGaauCCa -5' |
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9742 | 3' | -48.4 | NC_002577.1 | + | 116828 | 0.78 | 0.728076 |
Target: 5'- cUAAGGAACUACuGUGGUUCCUUAc-- -3' miRNA: 3'- -AUUCCUUGGUGuCAUCAAGGAAUcca -5' |
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9742 | 3' | -48.4 | NC_002577.1 | + | 116641 | 1.07 | 0.01855 |
Target: 5'- gUAAGGAACCACAGUAGUUCCUUAGGUa -3' miRNA: 3'- -AUUCCUUGGUGUCAUCAAGGAAUCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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