Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9759 | 3' | -48.6 | NC_002577.1 | + | 154840 | 0.66 | 0.999723 |
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Target: 5'- gCUAgCCGggaGAGAcgUGACCgcggUCACGu -3' miRNA: 3'- aGAUaGGCaagCUCU--ACUGGa---AGUGC- -5' |
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| 9759 | 3' | -48.6 | NC_002577.1 | + | 142497 | 0.66 | 0.999723 |
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Target: 5'- gCUAgCCGggaGAGAcgUGACCgcggUCACGu -3' miRNA: 3'- aGAUaGGCaagCUCU--ACUGGa---AGUGC- -5' |
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| 9759 | 3' | -48.6 | NC_002577.1 | + | 98736 | 0.67 | 0.999168 |
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Target: 5'- uUCUAUUCGg-CGAGAcccccaaggGACCUUUugGa -3' miRNA: 3'- -AGAUAGGCaaGCUCUa--------CUGGAAGugC- -5' |
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| 9759 | 3' | -48.6 | NC_002577.1 | + | 9603 | 0.68 | 0.997842 |
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Target: 5'- gUUAUCCGgaaUCGAGccgGACCUuUUACGu -3' miRNA: 3'- aGAUAGGCa--AGCUCua-CUGGA-AGUGC- -5' |
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| 9759 | 3' | -48.6 | NC_002577.1 | + | 56060 | 0.7 | 0.988542 |
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Target: 5'- -gUAUCUGUuugCGAGGUGAUCgcgacUCACGa -3' miRNA: 3'- agAUAGGCAa--GCUCUACUGGa----AGUGC- -5' |
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| 9759 | 3' | -48.6 | NC_002577.1 | + | 162928 | 1.12 | 0.012697 |
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Target: 5'- uUCUAUCCGUUCGAGAUGACCUUCACGg -3' miRNA: 3'- -AGAUAGGCAAGCUCUACUGGAAGUGC- -5' |
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| 9759 | 3' | -48.6 | NC_002577.1 | + | 134400 | 1.12 | 0.012697 |
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Target: 5'- uUCUAUCCGUUCGAGAUGACCUUCACGg -3' miRNA: 3'- -AGAUAGGCAAGCUCUACUGGAAGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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