Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9764 | 3' | -59.4 | NC_002577.1 | + | 137386 | 0.66 | 0.775625 |
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Target: 5'- --cUCGGGGGCG-CCAGgAGcgaaCUCUCg -3' miRNA: 3'- auaAGCCCCUGCaGGUCgUCc---GGGAG- -5' |
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| 9764 | 3' | -59.4 | NC_002577.1 | + | 149909 | 0.66 | 0.775625 |
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Target: 5'- ----aGGGGACGUgUAGUuuuucgcucaaAGGCCCa- -3' miRNA: 3'- auaagCCCCUGCAgGUCG-----------UCCGGGag -5' |
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| 9764 | 3' | -59.4 | NC_002577.1 | + | 157693 | 0.67 | 0.732225 |
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Target: 5'- gGggCGGGGAgggggagcgggcacuCGUUCuGCGGGCCC-Ca -3' miRNA: 3'- aUaaGCCCCU---------------GCAGGuCGUCCGGGaG- -5' |
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| 9764 | 3' | -59.4 | NC_002577.1 | + | 139645 | 0.67 | 0.732225 |
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Target: 5'- gGggCGGGGAgggggagcgggcacuCGUUCuGCGGGCCC-Ca -3' miRNA: 3'- aUaaGCCCCU---------------GCAGGuCGUCCGGGaG- -5' |
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| 9764 | 3' | -59.4 | NC_002577.1 | + | 159810 | 1.06 | 0.001981 |
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Target: 5'- gUAUUCGGGGACGUCCAGCAGGCCCUCc -3' miRNA: 3'- -AUAAGCCCCUGCAGGUCGUCCGGGAG- -5' |
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| 9764 | 3' | -59.4 | NC_002577.1 | + | 137528 | 1.06 | 0.001981 |
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Target: 5'- gUAUUCGGGGACGUCCAGCAGGCCCUCc -3' miRNA: 3'- -AUAAGCCCCUGCAGGUCGUCCGGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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