Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9766 | 5' | -58.3 | NC_002577.1 | + | 26216 | 0.66 | 0.801001 |
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Target: 5'- --uCACGGCuuugGGACUACGGGCgGCa-- -3' miRNA: 3'- uucGUGCCG----UCUGGUGCUCGgCGaac -5' |
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| 9766 | 5' | -58.3 | NC_002577.1 | + | 128827 | 0.66 | 0.809831 |
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Target: 5'- cGGCGCGGCGucgUCGCGcGCCGCg-- -3' miRNA: 3'- uUCGUGCCGUcu-GGUGCuCGGCGaac -5' |
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| 9766 | 5' | -58.3 | NC_002577.1 | + | 4786 | 0.66 | 0.809831 |
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Target: 5'- cGGCGCGGCGucgUCGCGcGCCGCg-- -3' miRNA: 3'- uUCGUGCCGUcu-GGUGCuCGGCGaac -5' |
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| 9766 | 5' | -58.3 | NC_002577.1 | + | 73816 | 0.66 | 0.809831 |
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Target: 5'- cAGUACGauGGGCCAUG-GCCGCUg- -3' miRNA: 3'- uUCGUGCcgUCUGGUGCuCGGCGAac -5' |
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| 9766 | 5' | -58.3 | NC_002577.1 | + | 134114 | 0.66 | 0.818501 |
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Target: 5'- -cGCGCGGCGGACC-CuGGCCa---- -3' miRNA: 3'- uuCGUGCCGUCUGGuGcUCGGcgaac -5' |
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| 9766 | 5' | -58.3 | NC_002577.1 | + | 163214 | 0.66 | 0.818501 |
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Target: 5'- -cGCGCGGCGGACC-CuGGCCa---- -3' miRNA: 3'- uuCGUGCCGUCUGGuGcUCGGcgaac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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