Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9766 | 5' | -58.3 | NC_002577.1 | + | 42434 | 0.73 | 0.412902 |
Target: 5'- -cGCGCGGguGACgACG-GCCGCa-- -3' miRNA: 3'- uuCGUGCCguCUGgUGCuCGGCGaac -5' |
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9766 | 5' | -58.3 | NC_002577.1 | + | 26216 | 0.66 | 0.801001 |
Target: 5'- --uCACGGCuuugGGACUACGGGCgGCa-- -3' miRNA: 3'- uucGUGCCG----UCUGGUGCUCGgCGaac -5' |
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9766 | 5' | -58.3 | NC_002577.1 | + | 8166 | 0.67 | 0.753789 |
Target: 5'- -uGCGCGGuCGGAUCGCGAGguuaacucccugcCCGCg-- -3' miRNA: 3'- uuCGUGCC-GUCUGGUGCUC-------------GGCGaac -5' |
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9766 | 5' | -58.3 | NC_002577.1 | + | 7840 | 0.68 | 0.665488 |
Target: 5'- -uGCGCGGCGGACaUACGcAGuuGCg-- -3' miRNA: 3'- uuCGUGCCGUCUG-GUGC-UCggCGaac -5' |
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9766 | 5' | -58.3 | NC_002577.1 | + | 4786 | 0.66 | 0.809831 |
Target: 5'- cGGCGCGGCGucgUCGCGcGCCGCg-- -3' miRNA: 3'- uUCGUGCCGUcu-GGUGCuCGGCGaac -5' |
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9766 | 5' | -58.3 | NC_002577.1 | + | 226 | 0.72 | 0.439405 |
Target: 5'- gGGGUGCGGCAGcCCGCGGGCUGa--- -3' miRNA: 3'- -UUCGUGCCGUCuGGUGCUCGGCgaac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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