Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9767 | 5' | -54.2 | NC_002577.1 | + | 543 | 0.66 | 0.948365 |
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Target: 5'- aGGGCCUCUCggggUCGGCG--UCa--GGACu -3' miRNA: 3'- -CCCGGAGAG----AGCCGUcaAGcuaUCUG- -5' |
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| 9767 | 5' | -54.2 | NC_002577.1 | + | 133071 | 0.66 | 0.948365 |
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Target: 5'- aGGGCCUCUCggggUCGGCG--UCa--GGACu -3' miRNA: 3'- -CCCGGAGAG----AGCCGUcaAGcuaUCUG- -5' |
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| 9767 | 5' | -54.2 | NC_002577.1 | + | 10157 | 0.69 | 0.85739 |
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Target: 5'- cGGCCUCUCgaacgagagCGGgGGUggggcauggUCGGUGGAg -3' miRNA: 3'- cCCGGAGAGa--------GCCgUCA---------AGCUAUCUg -5' |
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| 9767 | 5' | -54.2 | NC_002577.1 | + | 123457 | 0.69 | 0.85739 |
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Target: 5'- cGGCCUCUCgaacgagagCGGgGGUggggcauggUCGGUGGAg -3' miRNA: 3'- cCCGGAGAGa--------GCCgUCA---------AGCUAUCUg -5' |
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| 9767 | 5' | -54.2 | NC_002577.1 | + | 111913 | 0.7 | 0.841441 |
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Target: 5'- uGGCCUaagacuaUCGGCAGaugaagCGAUGGGCa -3' miRNA: 3'- cCCGGAgag----AGCCGUCaa----GCUAUCUG- -5' |
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| 9767 | 5' | -54.2 | NC_002577.1 | + | 158999 | 0.71 | 0.789227 |
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Target: 5'- cGGGCC--UCUCGGCGGacacCGAcAGGCg -3' miRNA: 3'- -CCCGGagAGAGCCGUCaa--GCUaUCUG- -5' |
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| 9767 | 5' | -54.2 | NC_002577.1 | + | 138338 | 0.72 | 0.741709 |
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Target: 5'- cGGGCC--UCUCGGCGGaacCGAcAGGCg -3' miRNA: 3'- -CCCGGagAGAGCCGUCaa-GCUaUCUG- -5' |
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| 9767 | 5' | -54.2 | NC_002577.1 | + | 157429 | 1.11 | 0.003582 |
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Target: 5'- gGGGCCUCUCUCGGCAGUUCGAUAGACc -3' miRNA: 3'- -CCCGGAGAGAGCCGUCAAGCUAUCUG- -5' |
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| 9767 | 5' | -54.2 | NC_002577.1 | + | 139908 | 1.11 | 0.003582 |
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Target: 5'- gGGGCCUCUCUCGGCAGUUCGAUAGACc -3' miRNA: 3'- -CCCGGAGAGAGCCGUCAAGCUAUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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