Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 9769 | 3' | -58.2 | NC_002577.1 | + | 154940 | 1.07 | 0.002419 |
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Target: 5'- uACUUGCCCCUAACGCACCGGCGUCCAc -3' miRNA: 3'- -UGAACGGGGAUUGCGUGGCCGCAGGU- -5' |
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| 9769 | 3' | -58.2 | NC_002577.1 | + | 155984 | 0.68 | 0.705204 |
|
Target: 5'- gGCcgUGCCCCcGACGC-CCGGUcggcagcccGUCCc -3' miRNA: 3'- -UGa-ACGGGGaUUGCGuGGCCG---------CAGGu -5' |
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| 9769 | 3' | -58.2 | NC_002577.1 | + | 157891 | 0.69 | 0.685353 |
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Target: 5'- uACUggacGCgCCUGACGCAgCGGagucgGUCCAg -3' miRNA: 3'- -UGAa---CGgGGAUUGCGUgGCCg----CAGGU- -5' |
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| 9769 | 3' | -58.2 | NC_002577.1 | + | 159201 | 0.72 | 0.496838 |
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Target: 5'- --aUGCCCCggAGCGCcugUCGGUGUCCGc -3' miRNA: 3'- ugaACGGGGa-UUGCGu--GGCCGCAGGU- -5' |
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| 9769 | 3' | -58.2 | NC_002577.1 | + | 160900 | 0.69 | 0.685353 |
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Target: 5'- gGCgaGCaCCUGuuuuGCGUACCGGCGcCCGg -3' miRNA: 3'- -UGaaCGgGGAU----UGCGUGGCCGCaGGU- -5' |
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| 9769 | 3' | -58.2 | NC_002577.1 | + | 162210 | 0.66 | 0.833532 |
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Target: 5'- --aUGCCCCgcGCGCACCuuCGcCCGg -3' miRNA: 3'- ugaACGGGGauUGCGUGGccGCaGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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