Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9769 | 3' | -58.2 | NC_002577.1 | + | 141353 | 0.68 | 0.705204 |
Target: 5'- gGCcgUGCCCCcGACGC-CCGGUcggcagcccGUCCc -3' miRNA: 3'- -UGa-ACGGGGaUUGCGuGGCCG---------CAGGu -5' |
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9769 | 3' | -58.2 | NC_002577.1 | + | 136437 | 0.69 | 0.685353 |
Target: 5'- gGCgaGCaCCUGuuuuGCGUACCGGCGcCCGg -3' miRNA: 3'- -UGaaCGgGGAU----UGCGUGGCCGCaGGU- -5' |
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9769 | 3' | -58.2 | NC_002577.1 | + | 157891 | 0.69 | 0.685353 |
Target: 5'- uACUggacGCgCCUGACGCAgCGGagucgGUCCAg -3' miRNA: 3'- -UGAa---CGgGGAUUGCGUgGCCg----CAGGU- -5' |
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9769 | 3' | -58.2 | NC_002577.1 | + | 159201 | 0.72 | 0.496838 |
Target: 5'- --aUGCCCCggAGCGCcugUCGGUGUCCGc -3' miRNA: 3'- ugaACGGGGa-UUGCGu--GGCCGCAGGU- -5' |
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9769 | 3' | -58.2 | NC_002577.1 | + | 41611 | 0.73 | 0.441593 |
Target: 5'- uCUUGUgCCUGuCGCGCCGGUGaCCAc -3' miRNA: 3'- uGAACGgGGAUuGCGUGGCCGCaGGU- -5' |
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9769 | 3' | -58.2 | NC_002577.1 | + | 82639 | 0.66 | 0.841624 |
Target: 5'- ---gGCCCU---CGaCGCCGGCGUUCGa -3' miRNA: 3'- ugaaCGGGGauuGC-GUGGCCGCAGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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