Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9770 | 5' | -53 | NC_002577.1 | + | 66888 | 0.66 | 0.971381 |
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Target: 5'- ----cGACGUGGCUaccggggGCGAAUAGGuGCUg -3' miRNA: 3'- aacaaCUGCACCGG-------CGUUUGUCU-CGA- -5' |
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| 9770 | 5' | -53 | NC_002577.1 | + | 17273 | 0.66 | 0.968654 |
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Target: 5'- -cGagGACGaUGGCCGCGGaaugucauGCcGAGCUa -3' miRNA: 3'- aaCaaCUGC-ACCGGCGUU--------UGuCUCGA- -5' |
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| 9770 | 5' | -53 | NC_002577.1 | + | 78180 | 0.66 | 0.96834 |
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Target: 5'- aUUGgcggUGAUGUGGuccucgcuucuacCCGCGgaGAUAGAGCa -3' miRNA: 3'- -AACa---ACUGCACC-------------GGCGU--UUGUCUCGa -5' |
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| 9770 | 5' | -53 | NC_002577.1 | + | 118288 | 0.66 | 0.965412 |
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Target: 5'- -cGUUGGCGacUGGCCcguGCGGACAacGGGUUa -3' miRNA: 3'- aaCAACUGC--ACCGG---CGUUUGU--CUCGA- -5' |
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| 9770 | 5' | -53 | NC_002577.1 | + | 86133 | 0.66 | 0.954292 |
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Target: 5'- -gGUaGGCuUGGCCgucagaucucuaGCAAACGGAGCg -3' miRNA: 3'- aaCAaCUGcACCGG------------CGUUUGUCUCGa -5' |
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| 9770 | 5' | -53 | NC_002577.1 | + | 154739 | 0.69 | 0.880089 |
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Target: 5'- -cGUUGGCGUGGCCaCGAGCucuGuuuGCg -3' miRNA: 3'- aaCAACUGCACCGGcGUUUGu--Cu--CGa -5' |
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| 9770 | 5' | -53 | NC_002577.1 | + | 50162 | 0.7 | 0.848924 |
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Target: 5'- aUGUcGaACGUGGCCGCuuGCGGAauaucGCa -3' miRNA: 3'- aACAaC-UGCACCGGCGuuUGUCU-----CGa -5' |
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| 9770 | 5' | -53 | NC_002577.1 | + | 81273 | 0.72 | 0.727249 |
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Target: 5'- -cGcUGAgGUGGCCGCGGGCGuGGCg -3' miRNA: 3'- aaCaACUgCACCGGCGUUUGUcUCGa -5' |
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| 9770 | 5' | -53 | NC_002577.1 | + | 154534 | 1.05 | 0.008571 |
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Target: 5'- uUUGUUGACGUGGCCGCAAACAGAGCUc -3' miRNA: 3'- -AACAACUGCACCGGCGUUUGUCUCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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