Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9771 | 3' | -53.2 | NC_002577.1 | + | 63457 | 0.66 | 0.970674 |
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Target: 5'- -uAGggGUUAGCCGAUAACGccccaucCGGAa -3' miRNA: 3'- ucUCuaCAAUCGGCUGUUGCc------GCCUa -5' |
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| 9771 | 3' | -53.2 | NC_002577.1 | + | 44173 | 0.66 | 0.960849 |
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Target: 5'- cGGGGA-GUUGGCCGGgAACGcGCGu-- -3' miRNA: 3'- -UCUCUaCAAUCGGCUgUUGC-CGCcua -5' |
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| 9771 | 3' | -53.2 | NC_002577.1 | + | 16306 | 0.67 | 0.944507 |
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Target: 5'- aAGGGAg--UGGCCGGCcuAACGGCauGGGUc -3' miRNA: 3'- -UCUCUacaAUCGGCUG--UUGCCG--CCUA- -5' |
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| 9771 | 3' | -53.2 | NC_002577.1 | + | 33807 | 0.68 | 0.932828 |
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Target: 5'- gAGGGAcGUUGGCCGGCAucuucauAUucuucucuccuagaGGCGGAa -3' miRNA: 3'- -UCUCUaCAAUCGGCUGU-------UG--------------CCGCCUa -5' |
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| 9771 | 3' | -53.2 | NC_002577.1 | + | 40339 | 0.68 | 0.918552 |
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Target: 5'- uGAG-UGUUuGUCGGCAGCGG-GGAUa -3' miRNA: 3'- uCUCuACAAuCGGCUGUUGCCgCCUA- -5' |
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| 9771 | 3' | -53.2 | NC_002577.1 | + | 160592 | 0.71 | 0.805253 |
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Target: 5'- gGGGGGccuUGUgcAGCUGGCGACaGGCGGAg -3' miRNA: 3'- -UCUCU---ACAa-UCGGCUGUUG-CCGCCUa -5' |
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| 9771 | 3' | -53.2 | NC_002577.1 | + | 136745 | 0.71 | 0.805253 |
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Target: 5'- gGGGGGccuUGUgcAGCUGGCGACaGGCGGAg -3' miRNA: 3'- -UCUCU---ACAa-UCGGCUGUUG-CCGCCUa -5' |
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| 9771 | 3' | -53.2 | NC_002577.1 | + | 141447 | 1.05 | 0.009144 |
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Target: 5'- uAGAGAUGUUAGCCGACAACGGCGGAUu -3' miRNA: 3'- -UCUCUACAAUCGGCUGUUGCCGCCUA- -5' |
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| 9771 | 3' | -53.2 | NC_002577.1 | + | 155890 | 1.05 | 0.009144 |
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Target: 5'- uAGAGAUGUUAGCCGACAACGGCGGAUu -3' miRNA: 3'- -UCUCUACAAUCGGCUGUUGCCGCCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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