Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9772 | 5' | -61.8 | NC_002577.1 | + | 101309 | 0.7 | 0.435769 |
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Target: 5'- uGCGGCUCgguCCGaUGCGGGCgaaGGCUccGCa -3' miRNA: 3'- -CGCCGAG---GGCaGCGCCCG---UCGGaaCG- -5' |
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| 9772 | 5' | -61.8 | NC_002577.1 | + | 80335 | 0.71 | 0.418816 |
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Target: 5'- --aGUUCCCGcCGCGGGCAuCCgcgUGCc -3' miRNA: 3'- cgcCGAGGGCaGCGCCCGUcGGa--ACG- -5' |
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| 9772 | 5' | -61.8 | NC_002577.1 | + | 139837 | 0.72 | 0.340397 |
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Target: 5'- cCGGCUCCUGgggcaGCGGGCGaCCUUucGCg -3' miRNA: 3'- cGCCGAGGGCag---CGCCCGUcGGAA--CG- -5' |
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| 9772 | 5' | -61.8 | NC_002577.1 | + | 157500 | 0.72 | 0.340397 |
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Target: 5'- cCGGCUCCUGgggcaGCGGGCGaCCUUucGCg -3' miRNA: 3'- cGCCGAGGGCag---CGCCCGUcGGAA--CG- -5' |
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| 9772 | 5' | -61.8 | NC_002577.1 | + | 141294 | 1.12 | 0.000638 |
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Target: 5'- cGCGGCUCCCGUCGCGGGCAGCCUUGCc -3' miRNA: 3'- -CGCCGAGGGCAGCGCCCGUCGGAACG- -5' |
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| 9772 | 5' | -61.8 | NC_002577.1 | + | 156044 | 1.12 | 0.000638 |
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Target: 5'- cGCGGCUCCCGUCGCGGGCAGCCUUGCc -3' miRNA: 3'- -CGCCGAGGGCAGCGCCCGUCGGAACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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