Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9789 | 3' | -53 | NC_002593.1 | + | 57708 | 0.66 | 0.935924 |
Target: 5'- --gUCGGCGGUGGGGUCGgCGGUg-- -3' miRNA: 3'- gagAGCUGCCGUCCUAGUgGUUAgac -5' |
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9789 | 3' | -53 | NC_002593.1 | + | 57684 | 0.66 | 0.935924 |
Target: 5'- --gUCGGCGGUGGGGUCGgCGGUg-- -3' miRNA: 3'- gagAGCUGCCGUCCUAGUgGUUAgac -5' |
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9789 | 3' | -53 | NC_002593.1 | + | 29566 | 0.67 | 0.893551 |
Target: 5'- -gUUCGACGGCgAGGGUCGC--GUCg- -3' miRNA: 3'- gaGAGCUGCCG-UCCUAGUGguUAGac -5' |
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9789 | 3' | -53 | NC_002593.1 | + | 13642 | 1.09 | 0.003244 |
Target: 5'- gCUCUCGACGGCAGGAUCACCAAUCUGg -3' miRNA: 3'- -GAGAGCUGCCGUCCUAGUGGUUAGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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