Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9790 | 5' | -52.1 | NC_002593.1 | + | 13841 | 1.09 | 0.003826 |
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Target: 5'- cGGAUCCAACCCUUGCAACCCCAACAAc -3' miRNA: 3'- -CCUAGGUUGGGAACGUUGGGGUUGUU- -5' |
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| 9790 | 5' | -52.1 | NC_002593.1 | + | 56938 | 0.67 | 0.898909 |
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Target: 5'- uGAUCCAuauauaucaACCCUaaucuauugcUGCAACCCCuguCGu -3' miRNA: 3'- cCUAGGU---------UGGGA----------ACGUUGGGGuu-GUu -5' |
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| 9790 | 5' | -52.1 | NC_002593.1 | + | 13756 | 0.67 | 0.924317 |
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Target: 5'- ---cCCAACCCcacCAACCCCGugGAu -3' miRNA: 3'- ccuaGGUUGGGaacGUUGGGGUugUU- -5' |
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| 9790 | 5' | -52.1 | NC_002593.1 | + | 13734 | 0.66 | 0.92999 |
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Target: 5'- uGGAUCCGcaGCCggUGUugAACCCCGGgGGa -3' miRNA: 3'- -CCUAGGU--UGGgaACG--UUGGGGUUgUU- -5' |
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| 9790 | 5' | -52.1 | NC_002593.1 | + | 73896 | 0.66 | 0.940517 |
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Target: 5'- aGGggCCAGCaCCUUGCcGCCCguGUAAu -3' miRNA: 3'- -CCuaGGUUG-GGAACGuUGGGguUGUU- -5' |
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| 9790 | 5' | -52.1 | NC_002593.1 | + | 61556 | 0.66 | 0.954288 |
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Target: 5'- uGcgCCGACCCUgugcuccauUGCAgcaaagacGCCuCCAACGAc -3' miRNA: 3'- cCuaGGUUGGGA---------ACGU--------UGG-GGUUGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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