Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9792 | 3' | -47.3 | NC_002593.1 | + | 18173 | 0.96 | 0.076381 |
Target: 5'- cUAGAAA-UGAUGAUCGAGGCGAAGGUu -3' miRNA: 3'- -AUCUUUaACUACUAGCUCCGCUUCCA- -5' |
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9792 | 3' | -47.3 | NC_002593.1 | + | 23908 | 0.71 | 0.954419 |
Target: 5'- cUGGAGGgugguugUGGUGGUCGAGGCGcAGu- -3' miRNA: 3'- -AUCUUUa------ACUACUAGCUCCGCuUCca -5' |
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9792 | 3' | -47.3 | NC_002593.1 | + | 37290 | 0.67 | 0.996074 |
Target: 5'- gUAGGcuguGUUGcggcGUGAUCGGGGUGAAGcGg -3' miRNA: 3'- -AUCUu---UAAC----UACUAGCUCCGCUUC-Ca -5' |
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9792 | 3' | -47.3 | NC_002593.1 | + | 57607 | 0.67 | 0.996074 |
Target: 5'- -uGGAGUcGGUGGUgGAGGaagaGggGGUg -3' miRNA: 3'- auCUUUAaCUACUAgCUCCg---CuuCCA- -5' |
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9792 | 3' | -47.3 | NC_002593.1 | + | 57812 | 0.73 | 0.889395 |
Target: 5'- gAGGAggUGGUGGUgGAGGUGGuggAGGUg -3' miRNA: 3'- aUCUUuaACUACUAgCUCCGCU---UCCA- -5' |
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9792 | 3' | -47.3 | NC_002593.1 | + | 57860 | 0.67 | 0.995356 |
Target: 5'- gAGGAGUUGGUGGaggggUCGGugguggugucGGUGGAGGa -3' miRNA: 3'- aUCUUUAACUACU-----AGCU----------CCGCUUCCa -5' |
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9792 | 3' | -47.3 | NC_002593.1 | + | 68260 | 0.66 | 0.998099 |
Target: 5'- gAGGAggUGGUGGUUGAGGaagacgagccCGAagAGGUg -3' miRNA: 3'- aUCUUuaACUACUAGCUCC----------GCU--UCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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