miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
9792 3' -47.3 NC_002593.1 + 18173 0.96 0.076381
Target:  5'- cUAGAAA-UGAUGAUCGAGGCGAAGGUu -3'
miRNA:   3'- -AUCUUUaACUACUAGCUCCGCUUCCA- -5'
9792 3' -47.3 NC_002593.1 + 23908 0.71 0.954419
Target:  5'- cUGGAGGgugguugUGGUGGUCGAGGCGcAGu- -3'
miRNA:   3'- -AUCUUUa------ACUACUAGCUCCGCuUCca -5'
9792 3' -47.3 NC_002593.1 + 37290 0.67 0.996074
Target:  5'- gUAGGcuguGUUGcggcGUGAUCGGGGUGAAGcGg -3'
miRNA:   3'- -AUCUu---UAAC----UACUAGCUCCGCUUC-Ca -5'
9792 3' -47.3 NC_002593.1 + 57607 0.67 0.996074
Target:  5'- -uGGAGUcGGUGGUgGAGGaagaGggGGUg -3'
miRNA:   3'- auCUUUAaCUACUAgCUCCg---CuuCCA- -5'
9792 3' -47.3 NC_002593.1 + 57812 0.73 0.889395
Target:  5'- gAGGAggUGGUGGUgGAGGUGGuggAGGUg -3'
miRNA:   3'- aUCUUuaACUACUAgCUCCGCU---UCCA- -5'
9792 3' -47.3 NC_002593.1 + 57860 0.67 0.995356
Target:  5'- gAGGAGUUGGUGGaggggUCGGugguggugucGGUGGAGGa -3'
miRNA:   3'- aUCUUUAACUACU-----AGCU----------CCGCUUCCa -5'
9792 3' -47.3 NC_002593.1 + 68260 0.66 0.998099
Target:  5'- gAGGAggUGGUGGUUGAGGaagacgagccCGAagAGGUg -3'
miRNA:   3'- aUCUUuaACUACUAGCUCC----------GCU--UCCA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.