Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9795 | 3' | -59 | NC_002593.1 | + | 71417 | 0.66 | 0.626951 |
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Target: 5'- uUCGCUUGcGCGGaCGACGACugCg-- -3' miRNA: 3'- cGGCGGAC-CGCCgGUUGCUGugGaaa -5' |
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| 9795 | 3' | -59 | NC_002593.1 | + | 2778 | 0.67 | 0.595779 |
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Target: 5'- uUCGCC-GGCGGCUAACGAUuUCg-- -3' miRNA: 3'- cGGCGGaCCGCCGGUUGCUGuGGaaa -5' |
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| 9795 | 3' | -59 | NC_002593.1 | + | 36284 | 0.67 | 0.595779 |
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Target: 5'- cGUCGUCUucgGGCGGCggCGGCGuCACCUc- -3' miRNA: 3'- -CGGCGGA---CCGCCG--GUUGCuGUGGAaa -5' |
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| 9795 | 3' | -59 | NC_002593.1 | + | 35363 | 0.68 | 0.504478 |
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Target: 5'- cUCGCCUccGGUGG-CGACGACGCCc-- -3' miRNA: 3'- cGGCGGA--CCGCCgGUUGCUGUGGaaa -5' |
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| 9795 | 3' | -59 | NC_002593.1 | + | 5999 | 0.7 | 0.437842 |
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Target: 5'- gGCCGCCUGaaUGGUCAGucugucugUGACGCCUUUg -3' miRNA: 3'- -CGGCGGACc-GCCGGUU--------GCUGUGGAAA- -5' |
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| 9795 | 3' | -59 | NC_002593.1 | + | 37935 | 1.08 | 0.001017 |
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Target: 5'- cGCCGCCUGGCGGCCAACGACACCUUUg -3' miRNA: 3'- -CGGCGGACCGCCGGUUGCUGUGGAAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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