Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9796 | 5' | -53.5 | NC_002593.1 | + | 57684 | 0.66 | 0.929533 |
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Target: 5'- gUCGGCgGUgGggUCgGCgGUGGGgucGGUg -3' miRNA: 3'- aAGCCG-CAgCuuAGgCGgCACUU---UCG- -5' |
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| 9796 | 5' | -53.5 | NC_002593.1 | + | 1490 | 0.66 | 0.923969 |
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Target: 5'- aUCGGCGUCGG---CGCCu--AGAGCg -3' miRNA: 3'- aAGCCGCAGCUuagGCGGcacUUUCG- -5' |
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| 9796 | 5' | -53.5 | NC_002593.1 | + | 22716 | 0.67 | 0.86945 |
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Target: 5'- -aCGauuGCGUCGAcgUCGUCGUGGAucugacgGGCa -3' miRNA: 3'- aaGC---CGCAGCUuaGGCGGCACUU-------UCG- -5' |
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| 9796 | 5' | -53.5 | NC_002593.1 | + | 57720 | 0.7 | 0.732928 |
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Target: 5'- gUCGGCGgagGAGUCgGCgGUGGGgucGGCg -3' miRNA: 3'- aAGCCGCag-CUUAGgCGgCACUU---UCG- -5' |
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| 9796 | 5' | -53.5 | NC_002593.1 | + | 31625 | 0.7 | 0.731902 |
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Target: 5'- --aGGCaGUCGAcucugaaAUCgGCCGUGuuGGCg -3' miRNA: 3'- aagCCG-CAGCU-------UAGgCGGCACuuUCG- -5' |
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| 9796 | 5' | -53.5 | NC_002593.1 | + | 75821 | 0.7 | 0.722631 |
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Target: 5'- -aUGGCGUCGucUCCGUCGUcGAAucGCc -3' miRNA: 3'- aaGCCGCAGCuuAGGCGGCA-CUUu-CG- -5' |
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| 9796 | 5' | -53.5 | NC_002593.1 | + | 7405 | 0.73 | 0.584718 |
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Target: 5'- gUUGGCGUUGGcguUCCGCUGUccGAGCu -3' miRNA: 3'- aAGCCGCAGCUu--AGGCGGCAcuUUCG- -5' |
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| 9796 | 5' | -53.5 | NC_002593.1 | + | 49209 | 0.74 | 0.532289 |
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Target: 5'- cUCGGUGUCGAcgCCGCUaaGAucGCu -3' miRNA: 3'- aAGCCGCAGCUuaGGCGGcaCUuuCG- -5' |
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| 9796 | 5' | -53.5 | NC_002593.1 | + | 38690 | 1.1 | 0.002544 |
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Target: 5'- uUUCGGCGUCGAAUCCGCCGUGAAAGCg -3' miRNA: 3'- -AAGCCGCAGCUUAGGCGGCACUUUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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