Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 9798 | 3' | -59.2 | NC_002593.1 | + | 18032 | 0.73 | 0.258567 |
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Target: 5'- gGGGugGCGa--CCUCACCGGCGaCAUc -3' miRNA: 3'- -UCCugCGCgagGGAGUGGCUGC-GUAa -5' |
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| 9798 | 3' | -59.2 | NC_002593.1 | + | 21919 | 0.66 | 0.634556 |
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Target: 5'- cAGGGCGCGUUCUCgcgcacgaUACCGGuuUGCAUa -3' miRNA: 3'- -UCCUGCGCGAGGGa-------GUGGCU--GCGUAa -5' |
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| 9798 | 3' | -59.2 | NC_002593.1 | + | 38190 | 0.66 | 0.644934 |
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Target: 5'- aAGG-CGCGCUCgacaaUCUguCUGACGCGUUu -3' miRNA: 3'- -UCCuGCGCGAG-----GGAguGGCUGCGUAA- -5' |
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| 9798 | 3' | -59.2 | NC_002593.1 | + | 51740 | 0.71 | 0.374929 |
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Target: 5'- cAGGuCGCGCUCuCCgagaCGCCGuCGCAg- -3' miRNA: 3'- -UCCuGCGCGAG-GGa---GUGGCuGCGUaa -5' |
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| 9798 | 3' | -59.2 | NC_002593.1 | + | 51811 | 0.68 | 0.512026 |
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Target: 5'- uGGACcUGCUCCUUCugCGGgGCGa- -3' miRNA: 3'- uCCUGcGCGAGGGAGugGCUgCGUaa -5' |
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| 9798 | 3' | -59.2 | NC_002593.1 | + | 58166 | 0.66 | 0.675972 |
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Target: 5'- aGGGGCGUGC-UgCUCAgCGGCGUGUc -3' miRNA: 3'- -UCCUGCGCGaGgGAGUgGCUGCGUAa -5' |
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| 9798 | 3' | -59.2 | NC_002593.1 | + | 88359 | 1.04 | 0.001759 |
|
Target: 5'- uAGGACGCGCUCCCUCACCGACGCAUUc -3' miRNA: 3'- -UCCUGCGCGAGGGAGUGGCUGCGUAA- -5' |
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| 9798 | 3' | -59.2 | NC_002593.1 | + | 90060 | 0.68 | 0.540986 |
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Target: 5'- uAGGAacagucUGCGUUaucaucaCCCUCguGCCGACGCAUa -3' miRNA: 3'- -UCCU------GCGCGA-------GGGAG--UGGCUGCGUAa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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