Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9802 | 5' | -58.7 | NC_002593.1 | + | 30321 | 0.66 | 0.706896 |
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Target: 5'- gUGUCUCUCGuaugUCGAGUuguACGCGUuGCUGa -3' miRNA: 3'- -ACGGGGAGC----AGCUCA---UGUGCAcCGGC- -5' |
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| 9802 | 5' | -58.7 | NC_002593.1 | + | 53751 | 0.67 | 0.666362 |
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Target: 5'- -aCUCCUUGUCGGGgcacACACGuUGGCUc -3' miRNA: 3'- acGGGGAGCAGCUCa---UGUGC-ACCGGc -5' |
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| 9802 | 5' | -58.7 | NC_002593.1 | + | 37902 | 0.69 | 0.55413 |
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Target: 5'- uUGCUgUUcaCGUCGAGcgacacagAUACGUGGCCGa -3' miRNA: 3'- -ACGGgGA--GCAGCUCa-------UGUGCACCGGC- -5' |
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| 9802 | 5' | -58.7 | NC_002593.1 | + | 81487 | 0.69 | 0.52427 |
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Target: 5'- -aCUCC-CGUCGuGUGCACGUGacacGCCGg -3' miRNA: 3'- acGGGGaGCAGCuCAUGUGCAC----CGGC- -5' |
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| 9802 | 5' | -58.7 | NC_002593.1 | + | 31475 | 0.69 | 0.51445 |
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Target: 5'- gGUCUCaCGUCgGGGUGCGCGUGGUUa -3' miRNA: 3'- aCGGGGaGCAG-CUCAUGUGCACCGGc -5' |
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| 9802 | 5' | -58.7 | NC_002593.1 | + | 28778 | 1.08 | 0.001286 |
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Target: 5'- uUGCCCCUCGUCGAGUACACGUGGCCGu -3' miRNA: 3'- -ACGGGGAGCAGCUCAUGUGCACCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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