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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 9804 | 5' | -59.4 | NC_002593.1 | + | 83266 | 0.66 | 0.662193 |
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Target: 5'- gCUGCAGCGaauauuuuuaucuuaUCG-UUGCGGUcguaACCGAGGu -3' miRNA: 3'- -GACGUCGC---------------GGCaGACGCCG----UGGCUUC- -5' |
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| 9804 | 5' | -59.4 | NC_002593.1 | + | 37806 | 0.67 | 0.606465 |
|
Target: 5'- -cGCaaaGGUGUCGUUggccgccagGCGGCGCCGAc- -3' miRNA: 3'- gaCG---UCGCGGCAGa--------CGCCGUGGCUuc -5' |
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| 9804 | 5' | -59.4 | NC_002593.1 | + | 11171 | 0.67 | 0.616779 |
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Target: 5'- uUGCAGCGaguCUGUCUggaGCGGCAUCa--- -3' miRNA: 3'- gACGUCGC---GGCAGA---CGCCGUGGcuuc -5' |
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| 9804 | 5' | -59.4 | NC_002593.1 | + | 1154 | 0.69 | 0.47658 |
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Target: 5'- uUGCGGCGCaCGUCUcCGaGCACgGAu- -3' miRNA: 3'- gACGUCGCG-GCAGAcGC-CGUGgCUuc -5' |
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| 9804 | 5' | -59.4 | NC_002593.1 | + | 36274 | 0.7 | 0.421313 |
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Target: 5'- aUGCAGUGCuCGUCgucuucggGCGGCGgCGGc- -3' miRNA: 3'- gACGUCGCG-GCAGa-------CGCCGUgGCUuc -5' |
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| 9804 | 5' | -59.4 | NC_002593.1 | + | 30875 | 1.08 | 0.000986 |
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Target: 5'- aCUGCAGCGCCGUCUGCGGCACCGAAGc -3' miRNA: 3'- -GACGUCGCGGCAGACGCCGUGGCUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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