Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9805 | 5' | -50.6 | NC_002593.1 | + | 53168 | 0.66 | 0.984422 |
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Target: 5'- gGAGUUacggCGAUCGUGAACACG-UCAu- -3' miRNA: 3'- aCUUAG----GCUGGCGCUUGUGCaAGUcu -5' |
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| 9805 | 5' | -50.6 | NC_002593.1 | + | 70016 | 0.66 | 0.980237 |
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Target: 5'- aUGGGcCUGACCGCGucGGCGgCGUUUAGu -3' miRNA: 3'- -ACUUaGGCUGGCGC--UUGU-GCAAGUCu -5' |
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| 9805 | 5' | -50.6 | NC_002593.1 | + | 73462 | 0.67 | 0.972466 |
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Target: 5'- cUGAGUCCGGugaaCGCGuGCugGaaCGGAg -3' miRNA: 3'- -ACUUAGGCUg---GCGCuUGugCaaGUCU- -5' |
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| 9805 | 5' | -50.6 | NC_002593.1 | + | 70543 | 0.67 | 0.966189 |
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Target: 5'- gUGGAaCUG-CCGuCGGACACGaUCAGGa -3' miRNA: 3'- -ACUUaGGCuGGC-GCUUGUGCaAGUCU- -5' |
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| 9805 | 5' | -50.6 | NC_002593.1 | + | 38681 | 0.68 | 0.954983 |
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Target: 5'- cGAAUCCG-CCGUGAaaGCGCGccgUCGc- -3' miRNA: 3'- aCUUAGGCuGGCGCU--UGUGCa--AGUcu -5' |
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| 9805 | 5' | -50.6 | NC_002593.1 | + | 96877 | 0.68 | 0.946253 |
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Target: 5'- uUGAAUCUcaACUGCGAGCACG-UCAu- -3' miRNA: 3'- -ACUUAGGc-UGGCGCUUGUGCaAGUcu -5' |
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| 9805 | 5' | -50.6 | NC_002593.1 | + | 31185 | 1.1 | 0.006208 |
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Target: 5'- gUGAAUCCGACCGCGAACACGUUCAGAc -3' miRNA: 3'- -ACUUAGGCUGGCGCUUGUGCAAGUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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