Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9810 | 3' | -55.5 | NC_002593.1 | + | 65946 | 0.66 | 0.831877 |
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Target: 5'- uUCCGC-UUAUCGaacCCACCguuaGGCAGUg -3' miRNA: 3'- gAGGCGaAAUGGC---GGUGGa---UCGUCGu -5' |
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| 9810 | 3' | -55.5 | NC_002593.1 | + | 38678 | 0.66 | 0.823077 |
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Target: 5'- aUCCGCcgUgaaagcgcGCCGUCGCCUgcugcuagguGGCGGUAa -3' miRNA: 3'- gAGGCGaaA--------UGGCGGUGGA----------UCGUCGU- -5' |
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| 9810 | 3' | -55.5 | NC_002593.1 | + | 1097 | 0.67 | 0.766637 |
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Target: 5'- --aCGCaagUGCUGCCAUCgUGGCGGCu -3' miRNA: 3'- gagGCGaa-AUGGCGGUGG-AUCGUCGu -5' |
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| 9810 | 3' | -55.5 | NC_002593.1 | + | 37945 | 0.68 | 0.70552 |
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Target: 5'- ---aGCUggucGgCGCCGCCUGGCGGCc -3' miRNA: 3'- gaggCGAaa--UgGCGGUGGAUCGUCGu -5' |
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| 9810 | 3' | -55.5 | NC_002593.1 | + | 89670 | 0.7 | 0.609937 |
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Target: 5'- gCUCgCGUagUACgGCgGCUUGGCAGCAu -3' miRNA: 3'- -GAG-GCGaaAUGgCGgUGGAUCGUCGU- -5' |
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| 9810 | 3' | -55.5 | NC_002593.1 | + | 31162 | 0.7 | 0.588662 |
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Target: 5'- cCUCCGCUaUGCaCGuCCA-CUGGUAGCGc -3' miRNA: 3'- -GAGGCGAaAUG-GC-GGUgGAUCGUCGU- -5' |
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| 9810 | 3' | -55.5 | NC_002593.1 | + | 959 | 0.72 | 0.485475 |
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Target: 5'- -aUCGCUUagguacaGCCGCCACgaUGGCAGCAc -3' miRNA: 3'- gaGGCGAAa------UGGCGGUGg-AUCGUCGU- -5' |
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| 9810 | 3' | -55.5 | NC_002593.1 | + | 38526 | 1.09 | 0.001779 |
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Target: 5'- gCUCCGCUUUACCGCCACCUAGCAGCAg -3' miRNA: 3'- -GAGGCGAAAUGGCGGUGGAUCGUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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