Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9810 | 5' | -54.5 | NC_002593.1 | + | 4542 | 0.66 | 0.896071 |
Target: 5'- aCGCGCUaaaccuGCGaGCGGAguacgCGuCGCCc -3' miRNA: 3'- -GCGCGAaag---UGC-CGCCUaa---GCuGCGG- -5' |
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9810 | 5' | -54.5 | NC_002593.1 | + | 75694 | 0.67 | 0.874886 |
Target: 5'- gGCGa-UUCgACGaCGGAgaCGACGCCa -3' miRNA: 3'- gCGCgaAAG-UGCcGCCUaaGCUGCGG- -5' |
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9810 | 5' | -54.5 | NC_002593.1 | + | 11557 | 0.67 | 0.867363 |
Target: 5'- aGgGUacuagUCAUGGUGGAg--GACGCCa -3' miRNA: 3'- gCgCGaa---AGUGCCGCCUaagCUGCGG- -5' |
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9810 | 5' | -54.5 | NC_002593.1 | + | 37684 | 0.67 | 0.851656 |
Target: 5'- uGCGUgaugaGCGGCGGuaaaGGCGUCg -3' miRNA: 3'- gCGCGaaag-UGCCGCCuaagCUGCGG- -5' |
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9810 | 5' | -54.5 | NC_002593.1 | + | 99390 | 0.69 | 0.771572 |
Target: 5'- gGCGUaaUCACGGUGacgUUGugGCCg -3' miRNA: 3'- gCGCGaaAGUGCCGCcuaAGCugCGG- -5' |
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9810 | 5' | -54.5 | NC_002593.1 | + | 7943 | 0.69 | 0.751116 |
Target: 5'- uGCGuUUUUCGgGGCGGAUaaagugguagauuUCGACcCCa -3' miRNA: 3'- gCGC-GAAAGUgCCGCCUA-------------AGCUGcGG- -5' |
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9810 | 5' | -54.5 | NC_002593.1 | + | 31306 | 0.7 | 0.742195 |
Target: 5'- aCGUGU--UCGCGGuCGGAUucacuUCGACGUUc -3' miRNA: 3'- -GCGCGaaAGUGCC-GCCUA-----AGCUGCGG- -5' |
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9810 | 5' | -54.5 | NC_002593.1 | + | 30678 | 0.7 | 0.711909 |
Target: 5'- gGCGUUUUaCGacgUGGUGGA--CGACGCCg -3' miRNA: 3'- gCGCGAAA-GU---GCCGCCUaaGCUGCGG- -5' |
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9810 | 5' | -54.5 | NC_002593.1 | + | 36446 | 0.71 | 0.653862 |
Target: 5'- uCGCGCUUcUCGUGGCgugcuacaacaaugaGGAggUGACGCCg -3' miRNA: 3'- -GCGCGAA-AGUGCCG---------------CCUaaGCUGCGG- -5' |
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9810 | 5' | -54.5 | NC_002593.1 | + | 76247 | 0.71 | 0.639197 |
Target: 5'- gGCGUUUUCgucuACGGCGGcGUUCaACGCg -3' miRNA: 3'- gCGCGAAAG----UGCCGCC-UAAGcUGCGg -5' |
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9810 | 5' | -54.5 | NC_002593.1 | + | 34249 | 0.82 | 0.181983 |
Target: 5'- gGUGCUgaggcugCGCGGCGGAUUCGACGa- -3' miRNA: 3'- gCGCGAaa-----GUGCCGCCUAAGCUGCgg -5' |
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9810 | 5' | -54.5 | NC_002593.1 | + | 38560 | 1.13 | 0.001626 |
Target: 5'- gCGCGCUUUCACGGCGGAUUCGACGCCg -3' miRNA: 3'- -GCGCGAAAGUGCCGCCUAAGCUGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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