Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9820 | 5' | -55.2 | NC_002604.1 | + | 5901 | 1.12 | 0.00003 |
Target: 5'- gCGCUUAUCCCGCUCCACUACCGACCCu -3' miRNA: 3'- -GCGAAUAGGGCGAGGUGAUGGCUGGG- -5' |
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9820 | 5' | -55.2 | NC_002604.1 | + | 6565 | 0.66 | 0.178229 |
Target: 5'- gGCUUugauaGUCaCCGC-CCACcACCugaGACCCc -3' miRNA: 3'- gCGAA-----UAG-GGCGaGGUGaUGG---CUGGG- -5' |
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9820 | 5' | -55.2 | NC_002604.1 | + | 253 | 0.66 | 0.183925 |
Target: 5'- cCGC-UGUCCCaCUcCCAC--CCGGCCUg -3' miRNA: 3'- -GCGaAUAGGGcGA-GGUGauGGCUGGG- -5' |
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9820 | 5' | -55.2 | NC_002604.1 | + | 3504 | 0.66 | 0.186249 |
Target: 5'- -uCUUAaucCCCGUguucaaauggugaucUUCACUGCCGAUCCa -3' miRNA: 3'- gcGAAUa--GGGCG---------------AGGUGAUGGCUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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