miRNA: 3'- -CGAG-GGG------------UugCCUCUA-------AACUUCCU-CC- -5'
miRNA: 3'- cGAGGGGU-UG---------------CCUCUaaACUUCCUCC- -5'
miRNA: 3'- -CGAGGGGUUGCCUCUAAACUUCCUCC- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9845 | 3' | -50.9 | NC_002618.2 | + | 2769 | 0.67 | 0.172701 |
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Target: 5'- gGCUCgCCUugguguaucgcaAugGGAGAUgauucugUUGAGGGAuGGg -3' miRNA: 3'- -CGAG-GGG------------UugCCUCUA-------AACUUCCU-CC- -5' |
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| 9845 | 3' | -50.9 | NC_002618.2 | + | 3580 | 0.71 | 0.100655 |
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Target: 5'- cCUCCCCAcACguuugaaguugugaaGGAGG--UGGAGGAGGc -3' miRNA: 3'- cGAGGGGU-UG---------------CCUCUaaACUUCCUCC- -5' |
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| 9845 | 3' | -50.9 | NC_002618.2 | + | 2884 | 1.13 | 3.3e-05 |
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Target: 5'- uGCUCCCCAACGGAGAUUUGAAGGAGGu -3' miRNA: 3'- -CGAGGGGUUGCCUCUAAACUUCCUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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