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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
9848 | 5' | -52.6 | NC_002628.2 | + | 11666 | 0.69 | 0.535633 |
Target: 5'- uCCUCcuuaaGGGCCUgugaAUCCGUAUCCc -3' miRNA: 3'- -GGAGacuacCCCGGAg---UAGGUAUAGG- -5' |
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9848 | 5' | -52.6 | NC_002628.2 | + | 17508 | 0.69 | 0.480255 |
Target: 5'- cCCUgUGAUGuGGGCCUCGaauUCCc---CCu -3' miRNA: 3'- -GGAgACUAC-CCCGGAGU---AGGuauaGG- -5' |
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9848 | 5' | -52.6 | NC_002628.2 | + | 13595 | 1.14 | 0.000387 |
Target: 5'- uCCUCUGAUGGGGCCUCAUCCAUAUCCu -3' miRNA: 3'- -GGAGACUACCCCGGAGUAGGUAUAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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