miRNA display CGI


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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
9849 5' -50.2 NC_002628.2 + 15990 0.66 0.778451
Target:  5'- --aUCCAGGG-CAGGGACauaCUGUa- -3'
miRNA:   3'- aaaAGGUUCCaGUCCCUGca-GAUAga -5'
9849 5' -50.2 NC_002628.2 + 13913 0.67 0.733158
Target:  5'- ---cUCAAGGUCAGGGcCG-CUGUa- -3'
miRNA:   3'- aaaaGGUUCCAGUCCCuGCaGAUAga -5'
9849 5' -50.2 NC_002628.2 + 15314 1.05 0.002368
Target:  5'- cUUUUCCAAGGUCAGGGACGUCUAUCUu -3'
miRNA:   3'- -AAAAGGUUCCAGUCCCUGCAGAUAGA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.