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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9853 | 5' | -53 | NC_002628.2 | + | 2103 | 0.66 | 0.62604 |
Target: 5'- aCUGGAGaggUAUCUUUcGAGGGCGCgGauGGCc -3' miRNA: 3'- -GACCUU---GUAGGAA-CUUUCGCGgC--CCG- -5' |
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9853 | 5' | -53 | NC_002628.2 | + | 23702 | 0.67 | 0.57923 |
Target: 5'- -aGGAuaGCGUagcCCUUGAuuGUgucaccacGCCGGGCu -3' miRNA: 3'- gaCCU--UGUA---GGAACUuuCG--------CGGCCCG- -5' |
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9853 | 5' | -53 | NC_002628.2 | + | 2080 | 1.13 | 0.000358 |
Target: 5'- cCUGGAACAUCCUUGAAAGCGCCGGGCa -3' miRNA: 3'- -GACCUUGUAGGAACUUUCGCGGCCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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