Results 1 - 9 of 9 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9866 | 3' | -45.1 | NC_002641.1 | + | 68686 | 0.66 | 0.999996 |
Target: 5'- --gGUUCGGC-UAUAACAU-GAUCGUa -3' miRNA: 3'- caaUAAGUCGcAUGUUGUAuCUGGCA- -5' |
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9866 | 3' | -45.1 | NC_002641.1 | + | 5654 | 0.67 | 0.999957 |
Target: 5'- ----aUCAGCGcGCGGCGggccGACCGg -3' miRNA: 3'- caauaAGUCGCaUGUUGUau--CUGGCa -5' |
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9866 | 3' | -45.1 | NC_002641.1 | + | 118068 | 0.67 | 0.999957 |
Target: 5'- ----aUCAGCGcGCGGCGggccGACCGg -3' miRNA: 3'- caauaAGUCGCaUGUUGUau--CUGGCa -5' |
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9866 | 3' | -45.1 | NC_002641.1 | + | 87078 | 0.69 | 0.999629 |
Target: 5'- aUUGUUCGGCGgauucccgGCAGCAUGcACUGg -3' miRNA: 3'- cAAUAAGUCGCa-------UGUUGUAUcUGGCa -5' |
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9866 | 3' | -45.1 | NC_002641.1 | + | 107515 | 0.74 | 0.978034 |
Target: 5'- -aUAUUUAGCGUugaacguugaugcACAugGUAGACUGUg -3' miRNA: 3'- caAUAAGUCGCA-------------UGUugUAUCUGGCA- -5' |
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9866 | 3' | -45.1 | NC_002641.1 | + | 1745 | 0.81 | 0.797498 |
Target: 5'- --cAUUCAGCGgGCAAUGUAGACUGUg -3' miRNA: 3'- caaUAAGUCGCaUGUUGUAUCUGGCA- -5' |
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9866 | 3' | -45.1 | NC_002641.1 | + | 121977 | 0.81 | 0.797498 |
Target: 5'- --cAUUCAGCGgGCAAUGUAGACUGUg -3' miRNA: 3'- caaUAAGUCGCaUGUUGUAUCUGGCA- -5' |
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9866 | 3' | -45.1 | NC_002641.1 | + | 1468 | 1.08 | 0.037223 |
Target: 5'- aGUUAUUCAGCGUACAACAUAGACCGUc -3' miRNA: 3'- -CAAUAAGUCGCAUGUUGUAUCUGGCA- -5' |
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9866 | 3' | -45.1 | NC_002641.1 | + | 122255 | 1.08 | 0.037223 |
Target: 5'- aGUUAUUCAGCGUACAACAUAGACCGUc -3' miRNA: 3'- -CAAUAAGUCGCAUGUUGUAUCUGGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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