Results 1 - 11 of 11 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9866 | 5' | -56.2 | NC_002641.1 | + | 34374 | 0.68 | 0.777865 |
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Target: 5'- uUAACGGCacgggaaaAUGUU-CCCGCUGGGUc- -3' miRNA: 3'- -GUUGUCGg-------UGCAAcGGGCGACCCAaa -5' |
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| 9866 | 5' | -56.2 | NC_002641.1 | + | 136737 | 0.7 | 0.668185 |
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Target: 5'- aCGGCuGCCGCGUggacGUCCGC-GGGUg- -3' miRNA: 3'- -GUUGuCGGUGCAa---CGGGCGaCCCAaa -5' |
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| 9866 | 5' | -56.2 | NC_002641.1 | + | 145735 | 0.7 | 0.668185 |
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Target: 5'- aCGGCuGCCGCGUggacGUCCGC-GGGUg- -3' miRNA: 3'- -GUUGuCGGUGCAa---CGGGCGaCCCAaa -5' |
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| 9866 | 5' | -56.2 | NC_002641.1 | + | 2257 | 0.71 | 0.626741 |
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Target: 5'- uUAACAGCUACaUUGUCCGCaGGGa-- -3' miRNA: 3'- -GUUGUCGGUGcAACGGGCGaCCCaaa -5' |
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| 9866 | 5' | -56.2 | NC_002641.1 | + | 121466 | 0.71 | 0.626741 |
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Target: 5'- uUAACAGCUACaUUGUCCGCaGGGa-- -3' miRNA: 3'- -GUUGUCGGUGcAACGGGCGaCCCaaa -5' |
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| 9866 | 5' | -56.2 | NC_002641.1 | + | 1940 | 0.79 | 0.230324 |
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Target: 5'- uUAGCAGCUACGUUGUCCGC-GGGa-- -3' miRNA: 3'- -GUUGUCGGUGCAACGGGCGaCCCaaa -5' |
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| 9866 | 5' | -56.2 | NC_002641.1 | + | 121782 | 0.79 | 0.230324 |
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Target: 5'- uUAGCAGCUACGUUGUCCGC-GGGa-- -3' miRNA: 3'- -GUUGUCGGUGCAACGGGCGaCCCaaa -5' |
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| 9866 | 5' | -56.2 | NC_002641.1 | + | 1713 | 0.88 | 0.058607 |
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Target: 5'- uGACAGCUACaUUGCCCGCUGGGUUUc -3' miRNA: 3'- gUUGUCGGUGcAACGGGCGACCCAAA- -5' |
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| 9866 | 5' | -56.2 | NC_002641.1 | + | 122009 | 0.88 | 0.058607 |
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Target: 5'- uGACAGCUACaUUGCCCGCUGGGUUUc -3' miRNA: 3'- gUUGUCGGUGcAACGGGCGACCCAAA- -5' |
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| 9866 | 5' | -56.2 | NC_002641.1 | + | 1434 | 1.04 | 0.004759 |
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Target: 5'- gCAACAGCCACGUUGCCCGCUGGGUUUc -3' miRNA: 3'- -GUUGUCGGUGCAACGGGCGACCCAAA- -5' |
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| 9866 | 5' | -56.2 | NC_002641.1 | + | 122289 | 1.04 | 0.004759 |
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Target: 5'- gCAACAGCCACGUUGCCCGCUGGGUUUc -3' miRNA: 3'- -GUUGUCGGUGCAACGGGCGACCCAAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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