Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9868 | 3' | -51.8 | NC_002641.1 | + | 135398 | 0.66 | 0.98846 |
Target: 5'- uACGCCCACAugugACAUggaugUCCGGuguuuuggaccGAUGcGCg -3' miRNA: 3'- -UGCGGGUGU----UGUGa----AGGCC-----------CUAUaUG- -5' |
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9868 | 3' | -51.8 | NC_002641.1 | + | 8669 | 0.66 | 0.98846 |
Target: 5'- cACGCUCAUGACAUUUCau-GAUGUAUg -3' miRNA: 3'- -UGCGGGUGUUGUGAAGgccCUAUAUG- -5' |
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9868 | 3' | -51.8 | NC_002641.1 | + | 147074 | 0.66 | 0.98846 |
Target: 5'- uACGCCCACAugugACAUggaugUCCGGuguuuuggaccGAUGcGCg -3' miRNA: 3'- -UGCGGGUGU----UGUGa----AGGCC-----------CUAUaUG- -5' |
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9868 | 3' | -51.8 | NC_002641.1 | + | 136748 | 0.66 | 0.988311 |
Target: 5'- gGCGCguaccgacggcugCCGCGugGacgUCCGcGGGUGUACa -3' miRNA: 3'- -UGCG-------------GGUGUugUga-AGGC-CCUAUAUG- -5' |
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9868 | 3' | -51.8 | NC_002641.1 | + | 145724 | 0.66 | 0.988311 |
Target: 5'- gGCGCguaccgacggcugCCGCGugGacgUCCGcGGGUGUACa -3' miRNA: 3'- -UGCG-------------GGUGUugUga-AGGC-CCUAUAUG- -5' |
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9868 | 3' | -51.8 | NC_002641.1 | + | 21626 | 0.66 | 0.981283 |
Target: 5'- uUGUCCugGACGaa--CGGGAUGUAUa -3' miRNA: 3'- uGCGGGugUUGUgaagGCCCUAUAUG- -5' |
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9868 | 3' | -51.8 | NC_002641.1 | + | 127403 | 0.67 | 0.979055 |
Target: 5'- cGCGCCCGCAgggggggccGCAgUuuauaUCCGaGGcUGUACg -3' miRNA: 3'- -UGCGGGUGU---------UGUgA-----AGGC-CCuAUAUG- -5' |
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9868 | 3' | -51.8 | NC_002641.1 | + | 155069 | 0.67 | 0.979055 |
Target: 5'- cGCGCCCGCAgggggggccGCAgUuuauaUCCGaGGcUGUACg -3' miRNA: 3'- -UGCGGGUGU---------UGUgA-----AGGC-CCuAUAUG- -5' |
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9868 | 3' | -51.8 | NC_002641.1 | + | 103151 | 0.67 | 0.979055 |
Target: 5'- cACGCCCA-AGCGCUcagaagaCgCGGGAUAUGa -3' miRNA: 3'- -UGCGGGUgUUGUGAa------G-GCCCUAUAUg -5' |
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9868 | 3' | -51.8 | NC_002641.1 | + | 118233 | 0.67 | 0.976636 |
Target: 5'- gUGCCCuaucuGCAACGCUucucugcugUCCGGGu--UACu -3' miRNA: 3'- uGCGGG-----UGUUGUGA---------AGGCCCuauAUG- -5' |
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9868 | 3' | -51.8 | NC_002641.1 | + | 5489 | 0.67 | 0.974017 |
Target: 5'- gUGCCCuaucuGCAACGCUuccugcugUCCGGGu--UACu -3' miRNA: 3'- uGCGGG-----UGUUGUGA--------AGGCCCuauAUG- -5' |
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9868 | 3' | -51.8 | NC_002641.1 | + | 69505 | 0.67 | 0.971193 |
Target: 5'- gGCGUgauggugaCUGCGACAUUUCUGGGAUGc-- -3' miRNA: 3'- -UGCG--------GGUGUUGUGAAGGCCCUAUaug -5' |
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9868 | 3' | -51.8 | NC_002641.1 | + | 32310 | 0.67 | 0.968154 |
Target: 5'- uCGCUguCAACACaaacccUCCGGGGaAUACg -3' miRNA: 3'- uGCGGguGUUGUGa-----AGGCCCUaUAUG- -5' |
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9868 | 3' | -51.8 | NC_002641.1 | + | 82175 | 0.69 | 0.93893 |
Target: 5'- uGCGCCaACAuugcCGCUUCCGGacuuuucguucguuGAUAUACu -3' miRNA: 3'- -UGCGGgUGUu---GUGAAGGCC--------------CUAUAUG- -5' |
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9868 | 3' | -51.8 | NC_002641.1 | + | 129040 | 0.69 | 0.93544 |
Target: 5'- cCGCCgGCAGcCGC-UCCGGGGccUACg -3' miRNA: 3'- uGCGGgUGUU-GUGaAGGCCCUauAUG- -5' |
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9868 | 3' | -51.8 | NC_002641.1 | + | 153432 | 0.69 | 0.93544 |
Target: 5'- cCGCCgGCAGcCGC-UCCGGGGccUACg -3' miRNA: 3'- uGCGGgUGUU-GUGaAGGCCCUauAUG- -5' |
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9868 | 3' | -51.8 | NC_002641.1 | + | 86656 | 0.7 | 0.919077 |
Target: 5'- gGCGCUUaaGCGACGCUgcucauuaUCGGGAUAUAUc -3' miRNA: 3'- -UGCGGG--UGUUGUGAa-------GGCCCUAUAUG- -5' |
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9868 | 3' | -51.8 | NC_002641.1 | + | 107544 | 0.71 | 0.882487 |
Target: 5'- uAUGCCCauauugcgguacucgACAGCACaUUCGGGcAUGUGCu -3' miRNA: 3'- -UGCGGG---------------UGUUGUGaAGGCCC-UAUAUG- -5' |
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9868 | 3' | -51.8 | NC_002641.1 | + | 4231 | 0.75 | 0.696627 |
Target: 5'- gAUGUaCCGCcGCACUUCCGGGAUugACu -3' miRNA: 3'- -UGCG-GGUGuUGUGAAGGCCCUAuaUG- -5' |
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9868 | 3' | -51.8 | NC_002641.1 | + | 119491 | 0.75 | 0.696627 |
Target: 5'- gAUGUaCCGCcGCACUUCCGGGAUugACu -3' miRNA: 3'- -UGCG-GGUGuUGUGAAGGCCCUAuaUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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