Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9869 | 5' | -56.6 | NC_002641.1 | + | 58597 | 0.66 | 0.894092 |
Target: 5'- -gGGGCGcGAUGuAUCaCUCGCAGCcCa -3' miRNA: 3'- cgCCCGCuUUAC-UAGcGGGCGUCGaG- -5' |
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9869 | 5' | -56.6 | NC_002641.1 | + | 67874 | 0.66 | 0.894092 |
Target: 5'- -gGGGCGA---GAUCGaaCUCGCAGUUUg -3' miRNA: 3'- cgCCCGCUuuaCUAGC--GGGCGUCGAG- -5' |
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9869 | 5' | -56.6 | NC_002641.1 | + | 30157 | 0.66 | 0.873324 |
Target: 5'- cUGGGCGAucugacuagagAAUGuUCG-CCGCAGCg- -3' miRNA: 3'- cGCCCGCU-----------UUACuAGCgGGCGUCGag -5' |
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9869 | 5' | -56.6 | NC_002641.1 | + | 148952 | 0.68 | 0.791153 |
Target: 5'- cGCGGaagGCGGcauGUGAgcugcggCGCCUGCuGCUCc -3' miRNA: 3'- -CGCC---CGCUu--UACUa------GCGGGCGuCGAG- -5' |
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9869 | 5' | -56.6 | NC_002641.1 | + | 133521 | 0.68 | 0.791153 |
Target: 5'- cGCGGaagGCGGcauGUGAgcugcggCGCCUGCuGCUCc -3' miRNA: 3'- -CGCC---CGCUu--UACUa------GCGGGCGuCGAG- -5' |
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9869 | 5' | -56.6 | NC_002641.1 | + | 118269 | 0.68 | 0.772757 |
Target: 5'- aCGGGCccguuauccucGAGccGGUCGCCCGCcGCg- -3' miRNA: 3'- cGCCCG-----------CUUuaCUAGCGGGCGuCGag -5' |
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9869 | 5' | -56.6 | NC_002641.1 | + | 76938 | 0.69 | 0.724746 |
Target: 5'- gGUGGG-GGAAUG-UCGCCCGCgaagGGUUUa -3' miRNA: 3'- -CGCCCgCUUUACuAGCGGGCG----UCGAG- -5' |
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9869 | 5' | -56.6 | NC_002641.1 | + | 118579 | 0.74 | 0.438794 |
Target: 5'- uGCGGGCGAucauuUCGCCCGCA-CUa -3' miRNA: 3'- -CGCCCGCUuuacuAGCGGGCGUcGAg -5' |
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9869 | 5' | -56.6 | NC_002641.1 | + | 5143 | 0.74 | 0.438794 |
Target: 5'- uGCGGGCGAucauuUCGCCCGCA-CUa -3' miRNA: 3'- -CGCCCGCUuuacuAGCGGGCGUcGAg -5' |
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9869 | 5' | -56.6 | NC_002641.1 | + | 5324 | 1.12 | 0.001669 |
Target: 5'- uGCGGGCGAAAUGAUCGCCCGCAGCUCg -3' miRNA: 3'- -CGCCCGCUUUACUAGCGGGCGUCGAG- -5' |
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9869 | 5' | -56.6 | NC_002641.1 | + | 118399 | 1.12 | 0.001669 |
Target: 5'- uGCGGGCGAAAUGAUCGCCCGCAGCUCg -3' miRNA: 3'- -CGCCCGCUUUACUAGCGGGCGUCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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