Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9871 | 3' | -50.4 | NC_002641.1 | + | 20949 | 0.66 | 0.994942 |
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Target: 5'- cGCGCCucagcGAGCCAA---AAcGGCGUCGc -3' miRNA: 3'- aCGCGG-----CUCGGUUaucUUaUCGUAGU- -5' |
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| 9871 | 3' | -50.4 | NC_002641.1 | + | 127112 | 0.67 | 0.988146 |
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Target: 5'- uUGCGCC--GCCAcUAGGcaAGCAUCc -3' miRNA: 3'- -ACGCGGcuCGGUuAUCUuaUCGUAGu -5' |
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| 9871 | 3' | -50.4 | NC_002641.1 | + | 155361 | 0.67 | 0.988146 |
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Target: 5'- uUGCGCC--GCCAcUAGGcaAGCAUCc -3' miRNA: 3'- -ACGCGGcuCGGUuAUCUuaUCGUAGu -5' |
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| 9871 | 3' | -50.4 | NC_002641.1 | + | 52398 | 0.67 | 0.987835 |
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Target: 5'- gGCGCCGGGCCcgacac-UGGCGUaCAg -3' miRNA: 3'- aCGCGGCUCGGuuaucuuAUCGUA-GU- -5' |
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| 9871 | 3' | -50.4 | NC_002641.1 | + | 86506 | 0.68 | 0.980646 |
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Target: 5'- uUGCGCCGAuGCggCAGUA----GGCAUCAg -3' miRNA: 3'- -ACGCGGCU-CG--GUUAUcuuaUCGUAGU- -5' |
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| 9871 | 3' | -50.4 | NC_002641.1 | + | 7630 | 1.1 | 0.007465 |
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Target: 5'- aUGCGCCGAGCCAAUAGAAUAGCAUCAg -3' miRNA: 3'- -ACGCGGCUCGGUUAUCUUAUCGUAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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