miRNA: 3'- aAGGUACCGuGGGACA--CCC-----AGGGUuc -5'
miRNA: 3'- aAGGUACCGuGGGACA--CCC-----AGGGUuc -5'
miRNA: 3'- aaGGUACCGUGGGa-CACC-C--------AGGGUUC- -5'
miRNA: 3'- aaGGUACCGUGGGa-CACC-C--------AGGGUUC- -5'
miRNA: 3'- -AAGGUACCGUGGGACACCCAGGGUUC- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9871 | 5' | -58.2 | NC_002641.1 | + | 126589 | 0.67 | 0.75655 |
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Target: 5'- aUCCGUGGC-CCUUGUaaGGGaguguUCCCGc- -3' miRNA: 3'- aAGGUACCGuGGGACA--CCC-----AGGGUuc -5' |
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| 9871 | 5' | -58.2 | NC_002641.1 | + | 155883 | 0.67 | 0.75655 |
|
Target: 5'- aUCCGUGGC-CCUUGUaaGGGaguguUCCCGc- -3' miRNA: 3'- aAGGUACCGuGGGACA--CCC-----AGGGUuc -5' |
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| 9871 | 5' | -58.2 | NC_002641.1 | + | 126879 | 0.74 | 0.348059 |
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Target: 5'- -cCCAUGGCACCCccGUGGuGaucaacguUCCCGAGg -3' miRNA: 3'- aaGGUACCGUGGGa-CACC-C--------AGGGUUC- -5' |
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| 9871 | 5' | -58.2 | NC_002641.1 | + | 155594 | 0.74 | 0.348059 |
|
Target: 5'- -cCCAUGGCACCCccGUGGuGaucaacguUCCCGAGg -3' miRNA: 3'- aaGGUACCGUGGGa-CACC-C--------AGGGUUC- -5' |
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| 9871 | 5' | -58.2 | NC_002641.1 | + | 7596 | 1.06 | 0.002303 |
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Target: 5'- gUUCCAUGGCACCCUGUGGGUCCCAAGa -3' miRNA: 3'- -AAGGUACCGUGGGACACCCAGGGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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