Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9872 | 5' | -43.7 | NC_002641.1 | + | 99720 | 0.66 | 1 |
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Target: 5'- ---cAUGAGAgGCggGACGgGAUCGCc -3' miRNA: 3'- cuuaUACUUUaCGuaCUGC-CUAGUGa -5' |
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| 9872 | 5' | -43.7 | NC_002641.1 | + | 133666 | 0.66 | 0.999999 |
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Target: 5'- cGAGUGUGAGAgGa--GGCGGGUUGCg -3' miRNA: 3'- -CUUAUACUUUaCguaCUGCCUAGUGa -5' |
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| 9872 | 5' | -43.7 | NC_002641.1 | + | 91021 | 0.67 | 0.999999 |
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Target: 5'- -cGUGUGGGcgaauacAUaGCAUcGGCGGAUCGCa -3' miRNA: 3'- cuUAUACUU-------UA-CGUA-CUGCCUAGUGa -5' |
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| 9872 | 5' | -43.7 | NC_002641.1 | + | 148806 | 0.66 | 0.999999 |
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Target: 5'- cGAGUGUGAGAgGa--GGCGGGUUGCg -3' miRNA: 3'- -CUUAUACUUUaCguaCUGCCUAGUGa -5' |
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| 9872 | 5' | -43.7 | NC_002641.1 | + | 152543 | 0.67 | 0.999997 |
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Target: 5'- aGAAUcgGAuGUGCcagAUGACGaaGAUCGCg -3' miRNA: 3'- -CUUAuaCUuUACG---UACUGC--CUAGUGa -5' |
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| 9872 | 5' | -43.7 | NC_002641.1 | + | 129929 | 0.67 | 0.999997 |
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Target: 5'- aGAAUcgGAuGUGCcagAUGACGaaGAUCGCg -3' miRNA: 3'- -CUUAuaCUuUACG---UACUGC--CUAGUGa -5' |
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| 9872 | 5' | -43.7 | NC_002641.1 | + | 18520 | 0.7 | 0.999886 |
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Target: 5'- ---cAUGAAAUGCAUcGCGGAUauuaGCa -3' miRNA: 3'- cuuaUACUUUACGUAcUGCCUAg---UGa -5' |
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| 9872 | 5' | -43.7 | NC_002641.1 | + | 13625 | 0.96 | 0.236221 |
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Target: 5'- ----cUGAAAUGCAUGACGGAUCACUa -3' miRNA: 3'- cuuauACUUUACGUACUGCCUAGUGA- -5' |
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| 9872 | 5' | -43.7 | NC_002641.1 | + | 13772 | 1.1 | 0.038475 |
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Target: 5'- aGAAUAUGAAAUGCAUGACGGAUCACUa -3' miRNA: 3'- -CUUAUACUUUACGUACUGCCUAGUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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