Results 1 - 9 of 9 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9874 | 3' | -54.6 | NC_002641.1 | + | 135499 | 0.67 | 0.882243 |
Target: 5'- gGCUGucuCAcUUCCUCCCGgACCCCa- -3' miRNA: 3'- -UGAUuccGUuAAGGAGGGC-UGGGGau -5' |
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9874 | 3' | -54.6 | NC_002641.1 | + | 152680 | 0.67 | 0.882243 |
Target: 5'- uUUGAGGCG---UCUCCCG-CCCCg- -3' miRNA: 3'- uGAUUCCGUuaaGGAGGGCuGGGGau -5' |
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9874 | 3' | -54.6 | NC_002641.1 | + | 146973 | 0.67 | 0.882243 |
Target: 5'- gGCUGucuCAcUUCCUCCCGgACCCCa- -3' miRNA: 3'- -UGAUuccGUuAAGGAGGGC-UGGGGau -5' |
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9874 | 3' | -54.6 | NC_002641.1 | + | 154461 | 0.7 | 0.762498 |
Target: 5'- --cGGGGCAAUUUCUCCCcggcGACCCg-- -3' miRNA: 3'- ugaUUCCGUUAAGGAGGG----CUGGGgau -5' |
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9874 | 3' | -54.6 | NC_002641.1 | + | 138299 | 0.7 | 0.762498 |
Target: 5'- uGCUGAuGGCGg--CCUCCCGGCUCUc- -3' miRNA: 3'- -UGAUU-CCGUuaaGGAGGGCUGGGGau -5' |
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9874 | 3' | -54.6 | NC_002641.1 | + | 128011 | 0.7 | 0.762498 |
Target: 5'- --cGGGGCAAUUUCUCCCcggcGACCCg-- -3' miRNA: 3'- ugaUUCCGUUAAGGAGGG----CUGGGgau -5' |
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9874 | 3' | -54.6 | NC_002641.1 | + | 129793 | 0.76 | 0.458507 |
Target: 5'- uUUGAGGCG---UCUCCCGGCCCCg- -3' miRNA: 3'- uGAUUCCGUuaaGGAGGGCUGGGGau -5' |
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9874 | 3' | -54.6 | NC_002641.1 | + | 139560 | 0.76 | 0.458507 |
Target: 5'- cACUAGGGCAAUUCgCUCgCgCGACUCCa- -3' miRNA: 3'- -UGAUUCCGUUAAG-GAG-G-GCUGGGGau -5' |
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9874 | 3' | -54.6 | NC_002641.1 | + | 37027 | 1.06 | 0.005192 |
Target: 5'- gACUAAGGCAAUUCCUCCCGACCCCUAc -3' miRNA: 3'- -UGAUUCCGUUAAGGAGGGCUGGGGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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