Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9874 | 5' | -53.9 | NC_002641.1 | + | 155754 | 0.66 | 0.958238 |
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Target: 5'- ---gGGGAUcCGGGGGGAguaccguguucccGCACAUGUa -3' miRNA: 3'- cagaCUCUGaGCCCUCCU-------------UGUGUGCG- -5' |
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| 9874 | 5' | -53.9 | NC_002641.1 | + | 147970 | 0.67 | 0.9465 |
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Target: 5'- aUCUGAGAUaCGGGcGGGggUugGgGUc -3' miRNA: 3'- cAGACUCUGaGCCC-UCCuuGugUgCG- -5' |
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| 9874 | 5' | -53.9 | NC_002641.1 | + | 134503 | 0.67 | 0.9465 |
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Target: 5'- aUCUGAGAUaCGGGcGGGggUugGgGUc -3' miRNA: 3'- cAGACUCUGaGCCC-UCCuuGugUgCG- -5' |
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| 9874 | 5' | -53.9 | NC_002641.1 | + | 18699 | 0.68 | 0.909903 |
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Target: 5'- uGUCUGAucUUUGGGAacaGGAAUugAUGCg -3' miRNA: 3'- -CAGACUcuGAGCCCU---CCUUGugUGCG- -5' |
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| 9874 | 5' | -53.9 | NC_002641.1 | + | 104936 | 0.69 | 0.897283 |
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Target: 5'- aUUUGGcGCgaagCGGGAGGAAUcauaaACACGCc -3' miRNA: 3'- cAGACUcUGa---GCCCUCCUUG-----UGUGCG- -5' |
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| 9874 | 5' | -53.9 | NC_002641.1 | + | 36991 | 1.11 | 0.003941 |
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Target: 5'- gGUCUGAGACUCGGGAGGAACACACGCc -3' miRNA: 3'- -CAGACUCUGAGCCCUCCUUGUGUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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