Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9875 | 3' | -50.8 | NC_002641.1 | + | 130085 | 0.66 | 0.994178 |
Target: 5'- cGCuACAUCCgGcucugAGCGGcgcCAUCgAUCCg -3' miRNA: 3'- aCGuUGUAGGgU-----UCGCCa--GUAG-UAGG- -5' |
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9875 | 3' | -50.8 | NC_002641.1 | + | 152388 | 0.66 | 0.994178 |
Target: 5'- cGCuACAUCCgGcucugAGCGGcgcCAUCgAUCCg -3' miRNA: 3'- aCGuUGUAGGgU-----UCGCCa--GUAG-UAGG- -5' |
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9875 | 3' | -50.8 | NC_002641.1 | + | 31518 | 0.66 | 0.993261 |
Target: 5'- aGUAGCA-CCCAAGCuucGUUGUugcCAUCCa -3' miRNA: 3'- aCGUUGUaGGGUUCGc--CAGUA---GUAGG- -5' |
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9875 | 3' | -50.8 | NC_002641.1 | + | 12771 | 0.66 | 0.992232 |
Target: 5'- gGCAgacGCGUCCgAuGgGGUCGUagcgaAUCCa -3' miRNA: 3'- aCGU---UGUAGGgUuCgCCAGUAg----UAGG- -5' |
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9875 | 3' | -50.8 | NC_002641.1 | + | 25023 | 0.67 | 0.986815 |
Target: 5'- aGauauCGUCCCuuuAGCGG-CGUCGUCg -3' miRNA: 3'- aCguu-GUAGGGu--UCGCCaGUAGUAGg -5' |
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9875 | 3' | -50.8 | NC_002641.1 | + | 45445 | 0.67 | 0.985089 |
Target: 5'- aUGCAuuuCAUCCaCGAGCcGGUCcUCugcugCCa -3' miRNA: 3'- -ACGUu--GUAGG-GUUCG-CCAGuAGua---GG- -5' |
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9875 | 3' | -50.8 | NC_002641.1 | + | 120578 | 0.67 | 0.985089 |
Target: 5'- gGUcAUGUCCCGAGaUGGUCGUC-UCg -3' miRNA: 3'- aCGuUGUAGGGUUC-GCCAGUAGuAGg -5' |
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9875 | 3' | -50.8 | NC_002641.1 | + | 3145 | 0.67 | 0.985089 |
Target: 5'- gGUcAUGUCCCGAGaUGGUCGUC-UCg -3' miRNA: 3'- aCGuUGUAGGGUUC-GCCAGUAGuAGg -5' |
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9875 | 3' | -50.8 | NC_002641.1 | + | 103681 | 0.67 | 0.984908 |
Target: 5'- aUGUAACGUUUCuuuauuuGAGCGGUCAcaaaUCAgaagCCa -3' miRNA: 3'- -ACGUUGUAGGG-------UUCGCCAGU----AGUa---GG- -5' |
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9875 | 3' | -50.8 | NC_002641.1 | + | 152176 | 0.67 | 0.983197 |
Target: 5'- cGCGGuCGUCCCGuGCG-UCGUacaCGUCCa -3' miRNA: 3'- aCGUU-GUAGGGUuCGCcAGUA---GUAGG- -5' |
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9875 | 3' | -50.8 | NC_002641.1 | + | 130296 | 0.67 | 0.983197 |
Target: 5'- cGCGGuCGUCCCGuGCG-UCGUacaCGUCCa -3' miRNA: 3'- aCGUU-GUAGGGUuCGCcAGUA---GUAGG- -5' |
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9875 | 3' | -50.8 | NC_002641.1 | + | 109972 | 0.68 | 0.976427 |
Target: 5'- --aGACAUgCUAcGGCGGUCAUC-UCCc -3' miRNA: 3'- acgUUGUAgGGU-UCGCCAGUAGuAGG- -5' |
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9875 | 3' | -50.8 | NC_002641.1 | + | 60433 | 0.7 | 0.948992 |
Target: 5'- aGCAAUAuUCCCucGUGGUUuUCAUCa -3' miRNA: 3'- aCGUUGU-AGGGuuCGCCAGuAGUAGg -5' |
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9875 | 3' | -50.8 | NC_002641.1 | + | 70656 | 0.7 | 0.929467 |
Target: 5'- gGCGGCAuuucaagcgccUCCgGAGCGGUUugguguUCGUCUa -3' miRNA: 3'- aCGUUGU-----------AGGgUUCGCCAGu-----AGUAGG- -5' |
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9875 | 3' | -50.8 | NC_002641.1 | + | 152861 | 0.71 | 0.912137 |
Target: 5'- gGCAGCA-CCCc-GCGuGUCggCAUCCg -3' miRNA: 3'- aCGUUGUaGGGuuCGC-CAGuaGUAGG- -5' |
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9875 | 3' | -50.8 | NC_002641.1 | + | 129611 | 0.71 | 0.912137 |
Target: 5'- gGCAGCA-CCCc-GCGuGUCggCAUCCg -3' miRNA: 3'- aCGUUGUaGGGuuCGC-CAGuaGUAGG- -5' |
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9875 | 3' | -50.8 | NC_002641.1 | + | 136032 | 0.72 | 0.885492 |
Target: 5'- aUGUGGCccaCCCAGGCGGUCcuGUCG-CCg -3' miRNA: 3'- -ACGUUGua-GGGUUCGCCAG--UAGUaGG- -5' |
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9875 | 3' | -50.8 | NC_002641.1 | + | 146441 | 0.72 | 0.885492 |
Target: 5'- aUGUGGCccaCCCAGGCGGUCcuGUCG-CCg -3' miRNA: 3'- -ACGUUGua-GGGUUCGCCAG--UAGUaGG- -5' |
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9875 | 3' | -50.8 | NC_002641.1 | + | 48871 | 0.74 | 0.77361 |
Target: 5'- gGUGugAUUCCAAGUGGUCAUUucccauaAUCCu -3' miRNA: 3'- aCGUugUAGGGUUCGCCAGUAG-------UAGG- -5' |
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9875 | 3' | -50.8 | NC_002641.1 | + | 38677 | 1.12 | 0.005696 |
Target: 5'- aUGCAACAUCCCAAGCGGUCAUCAUCCc -3' miRNA: 3'- -ACGUUGUAGGGUUCGCCAGUAGUAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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